Cloned (Comment) | Organism |
---|---|
two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme | Gallus gallus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme. The bactericidal activity against Escherichia coli of S6L-lysozyme and S7-lysozyme is greatly increased | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium | Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | two serine-rich heptapeptides, Ser-Ser-Ser-Lys-Ser-Ser-Ser (S6K) and Ser-Ser-Ser-Ser-Ser-Ser-Ser (S7) are fused to the C-terminus of chicken lysozyme by genetic modification. The cDNAs of S6K-lysozyme and S7-lysozyme are inserted into the expression vector of Pichia pastoris and secreted in the yeast cultivation medium. The secretion amounts of S6K-lysozyme and S7-lysozyme are about 60% of that of wild-type lysozyme | Gallus gallus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidoglycan + H2O | lysis of Micrococcus lysodeikticus cells | Gallus gallus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
76 | - |
mid-transition temperature is 76.3°C for S6K-lysozyme and 76.0°C for S7-lysozyme | Gallus gallus |
77 | - |
mid-transition temperature of wild-type enzyme | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
lysis of Micrococcus lysodeikticus cells | Gallus gallus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: wild-type enzyme shows about 80% of maximal activity, S6K-lysozyme and S7-lysozyme shows about 60% of maximal activity, pH 7.0: about 40% of maximal activity, wild-type enzyme, S6K-lysozyme and S7-lysozyme, lysis of Micrococcus lysodeikticus cells | Gallus gallus |