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Literature summary for 3.2.1.145 extracted from

  • Fujita, K.; Sakaguchi, T.; Sakamoto, A.; Shimokawa, M.; Kitahara, K.
    Bifidobacterium longum subsp. longum exo-beta-1,3-galactanase, an enzyme for the degradation of type II arabinogalactan (2014), Appl. Environ. Microbiol., 80, 4577-4584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.145

Cloned(Commentary)

Cloned (Comment) Organism
BLLJ_1840, phylogenetic tree, expresion as soluble enzyme in Escherichia coli Bifidobacterium longum

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity Bifidobacterium longum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic parameters of enzyme BLLJ_1840 activity on different substrates, overview Bifidobacterium longum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
106100
-
 sequence calculation Bifidobacterium longum

Organism

Organism UniProt Comment Textmining
Bifidobacterium longum A0A061M1M5 subsp. longum
-
Bifidobacterium longum JCM 1217 A0A061M1M5 subsp. longum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0139
-
 substrate beta-1,3-D-galactotetraose, pH 5.5, 40°C Bifidobacterium longum
0.0157
-
 substrate beta-1,3-D-galactopentaose, pH 5.5, 40°C Bifidobacterium longum
0.0188
-
 substrate beta-1,3-D-galactotriose, pH 5.5, 40°C Bifidobacterium longum
0.0218
-
 substrate beta-1,3-D-galactobiose, pH 5.5, 40°C Bifidobacterium longum
0.113
-
 substrate gum arabic, pH 5.5, 40°C Bifidobacterium longum
1.01
-
 substrate beta-1,3-galactan, pH 5.5, 40°C Bifidobacterium longum
1.17
-
 substrate larch wood arabinogalactan, pH 5.5, 40°C Bifidobacterium longum
1.28
-
 substrate dearabinosylated gum arabic, pH 5.5, 40°C Bifidobacterium longum
16.2
-
 substrate dearabinosylated larch wood arabinogalactan, pH 5.5, 40°C Bifidobacterium longum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-D-galactobiose + H2O best beta-1,3-galactan substrate Bifidobacterium longum 2 D-galactose
-
 ?
beta-1,3-D-galactobiose + H2O best beta-1,3-galactan substrate Bifidobacterium longum JCM 1217 2 D-galactose
-
 ?
beta-1,3-D-galactopentaose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
beta-1,3-D-galactopentaose + H2O
-
 Bifidobacterium longum JCM 1217 D-galactose + ?
-
 ?
beta-1,3-D-galactotetraose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
beta-1,3-D-galactotetraose + H2O
-
 Bifidobacterium longum JCM 1217 D-galactose + ?
-
 ?
beta-1,3-D-galactotriose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
dearabinosylated gum arabic + H2O
-
 Bifidobacterium longum ?
-
 ?
dearabinosylated larch wood arabinogalactan + H2O best substrate Bifidobacterium longum ?
-
 ?
gum arabic + H2O
-
 Bifidobacterium longum ?
-
 ?
gum arabic + H2O
-
 Bifidobacterium longum JCM 1217 ?
-
 ?
larch wood arabinogalactan + H2O
-
 Bifidobacterium longum ?
-
 ?
additional information the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview Bifidobacterium longum ?
-
 ?
additional information the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview Bifidobacterium longum JCM 1217 ?
-
 ?

Synonyms

Synonyms Comment Organism
BLLJ_1840
-
 Bifidobacterium longum
exo-beta-1,3-galactanase
-
 Bifidobacterium longum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Bifidobacterium longum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Bifidobacterium longum

Expression

Organism Comment Expression
Bifidobacterium longum the enzyme is strongly induced by larch wood arabinogalactan up

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic relationships of BLLJ_1840 Bifidobacterium longum
additional information domain structures of BLLJ_1840 comprising the GH43 catalytic domain, bacterial Ig-like domain, CBM32, and the LPXTG cell wall anchor domain (Anchor), as well as the signal peptide Bifidobacterium longum