Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | addition of galactose at a concentration of 2 mM to the reaction mixture does not affect the enzyme activity | Sphingomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Sphingomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingomonas sp. | - |
- |
- |
Sphingomonas sp. 24T | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Toyopearl 650-M column chromatography and Resource PHE column chromatography | Sphingomonas sp. |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.88 | - |
culture filtrate, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C | Sphingomonas sp. |
1.7 | - |
after 1.9fold purification, using beta-(1-3)-D-galactan as substrate, in 20 mM Na phosphate buffer (pH 6.0) at 37°C | Sphingomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-(1,3)-D-galactooligosaccharides + H2O | - |
Sphingomonas sp. | D-galactose + ? | - |
? | |
beta-(1,3)-D-galactooligosaccharides + H2O | - |
Sphingomonas sp. 24T | D-galactose + ? | - |
? | |
beta-(1,3)-galactobiose + H2O | - |
Sphingomonas sp. | D-galactose | - |
? | |
beta-(1,3)-galactobiose + H2O | - |
Sphingomonas sp. 24T | D-galactose | - |
? | |
beta-(1-3)-D-galactan + H2O | the enzyme releases approximately 60% of the total D-galactose in the substrate | Sphingomonas sp. | beta-(1-3)-D-galactan + D-galactose | - |
? | |
beta-(1-3)-D-galactan + H2O | the enzyme releases approximately 60% of the total D-galactose in the substrate | Sphingomonas sp. 24T | beta-(1-3)-D-galactan + D-galactose | - |
? | |
beta-1,3-galactotriose + H2O | - |
Sphingomonas sp. | D-galactose + beta-(1,3)-galactobiose | - |
? | |
beta-1,3-galactotriose + H2O | - |
Sphingomonas sp. 24T | D-galactose + beta-(1,3)-galactobiose | - |
? | |
gum arabic + H2O | weak substrate | Sphingomonas sp. | ? | - |
? | |
larch wood arabinogalactan + H2O | the enzyme bypasses the branching points of beta-1,3-galactan backbones in larch wood arabinogalactan | Sphingomonas sp. | D-galactose + beta-(1->6)-D-galactobiose + beta-(1->6)-D-galactotriose + O-alpha-L-arabinofuranosyl-(1->3)-O-beta-D-galactopyranosyl-(1->6)-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + O-beta-D-galactopyranosyl-(1->6)-[O-alpha-L-arabinofuranosyl-(1->3)-]-O-beta-D-galactopyranosyl-(1->6)-D-galactopyranose + ? | - |
? | |
additional information | no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages | Sphingomonas sp. | ? | - |
? | |
additional information | no activity with lupin galactan, carob galactomannan, sugar beet L-arabinan, sugar beet debranched arabinan, wheat arabinoxylan, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl beta-D-xylopyranoside, 4-nitrophenyl alpha-L-arabinopyranoside, and 4-nitrophenyl alpha-L-arabinofuranoside. The enzyme is not active on galactooligosaccharides with beta-1,4- or beta-1,6-linkages | Sphingomonas sp. 24T | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Sphingomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
Exo-1,3-Gal | - |
Sphingomonas sp. |
exo-beta-1,3-D-galactanase | - |
Sphingomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme is most active around 50°C | Sphingomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
the enzyme is stable at temperatures up to 40°C | Sphingomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | at 37°C | Sphingomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | more than 80% of the initial activity remains after 16 h of incubation at pH values from 3.0 to 10.0 | Sphingomonas sp. |