Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of the gene encoding the enzyme fused to a yeast secretion signal sequence under control of the alcohol oxidase promoter in Pichia pastoris | Irpex lacteus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45520 | - |
x * 45520, mature enzyme, sequence calculation | Irpex lacteus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Irpex lacteus | - |
- |
- |
Irpex lacteus NBRC 5367 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 0.7fold from Pichia pastoris cell culture medium by cation exchange chromatography and gel filtration | Irpex lacteus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.6 | - |
purified recombinant enzyme | Irpex lacteus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,3-galactan + H2O | best substrate, mode of action, overview | Irpex lacteus | ? | - |
? | |
beta-1,3-galactan + H2O | best substrate, mode of action, overview | Irpex lacteus NBRC 5367 | ? | - |
? | |
additional information | substrate specificity, the recombinant enzyme specifically hydrolyses beta-1,3-galactooligosacchrides, as an exo-beta-1,3-galactanase, with high activity for arabinogalactan-proteins from radish, hydrolyzing beta-1,3-galactan main chains, as well as beta-1,3-galactan. Products are beta-1,6-galactobiose, beta-1,6-galactotriose, alpha-arabinofuranosyl-1,3-galactosyl-beta-1,6-galatose with galactose from beta-1,3-galactans attached with and without beta-1,6-galatosyl branchesprepared from acacia gum, overview | Irpex lacteus | ? | - |
? | |
additional information | substrate specificity, the recombinant enzyme specifically hydrolyses beta-1,3-galactooligosacchrides, as an exo-beta-1,3-galactanase, with high activity for arabinogalactan-proteins from radish, hydrolyzing beta-1,3-galactan main chains, as well as beta-1,3-galactan. Products are beta-1,6-galactobiose, beta-1,6-galactotriose, alpha-arabinofuranosyl-1,3-galactosyl-beta-1,6-galatose with galactose from beta-1,3-galactans attached with and without beta-1,6-galatosyl branchesprepared from acacia gum, overview | Irpex lacteus NBRC 5367 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45520, mature enzyme, sequence calculation | Irpex lacteus |
Synonyms | Comment | Organism |
---|---|---|
exo-/beta-1,3-galactanase | - |
Irpex lacteus |
Il1,3Gal | - |
Irpex lacteus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | Irpex lacteus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | activity range, temperature profile, recombinant enzyme, overview | Irpex lacteus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
recombinant enzyme | Irpex lacteus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | activity range, pH profile, recombinant enzyme, overview | Irpex lacteus |