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Literature summary for 3.2.1.145 extracted from

  • Kotake, T.; Dina, S.; Konishi, T.; Kaneko, S.; Igarashi, K.; Samejima, M.; Watanabe, Y.; Kimura, K.; Tsumuraya, Y.
    Molecular cloning of a b-galactosidase from radish that specifically hydrolyzes beta-(1->3)- and beta-(1->6)-galactosyl residues of arabinogalactan protein (2005), Plant Physiol., 138, 1563-1576.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.145

Cloned(Commentary)

Cloned (Comment) Organism
-
 Raphanus sativus

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, almost complete inhibition Raphanus sativus
iodoacetic acid 1 mM, about 80% inhibition Raphanus sativus
additional information not inhibitory: Mn2+, Fe3+, Cu2+, Mg2+, Ba2+, Ca2+, Co2+, Zn2+, Cd2+ Raphanus sativus
sodium dodecylsulfate 1 mM, about 80% inhibition Raphanus sativus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 p-nitrophenyl-beta-galactoside pH 4.0, 37°C Raphanus sativus
0.77
-
 beta-(1-3)-D-galactotriose pH 4.0, 37°C Raphanus sativus
1.77
-
 beta-(1-3)-galactobiose pH 4.0, 37°C Raphanus sativus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33654
-
 x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated Raphanus sativus
44981
-
 x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated Raphanus sativus

Organism

Organism UniProt Comment Textmining
Raphanus sativus Q6L619 recombinant enzyme
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Pichia pastoris Raphanus sativus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
64.9
-
 pH 4.0, 37°C Raphanus sativus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-(1-3)-D-galactan + H2O 8% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-(1-3)-D-galactan + D-galactose
-
 ?
beta-(1-3)-D-galactotriose + H2O 44% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-(1-3)-D-galactobiose + D-galactose
-
 ?
beta-(1-3)-galactobiose + H2O 32% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus D-galactose
-
 ?
beta-(1-6)-galactopentaose + H2O 50% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-(1-6)-D-galactotetraose + D-galactose
-
 ?
beta-(1-6)-galactotetraose + H2O 68% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-(1-6)-D-galactotriose + D-galactose
-
 ?
beta-(1-6)-galactotriose + H2O 49% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-(1-6)-D-galactobiose + D-galactose
-
 ?
beta-galactosido-(1-3)-beta-galactosido-(1-6)-galactoside + H2O 42% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-galactosido-(1-3)-beta-galactoside + D-galactose
-
 ?
beta-galactosido-(1-6)-beta-galactosido-(1-3)-galactoside + H2O 40% of the activity with p-nitrophenyl-beta-galactoside Raphanus sativus beta-galactosido-(1-6)-beta-galactoside + D-galactose
-
 ?
additional information specific hydrolysis of beta-1,3 and beta-1,6 galactooligosaccharides Raphanus sativus ?
-
 ?
p-nitrophenyl-beta-galactoside + H2O
-
 Raphanus sativus p-nitrophenol + beta-galactose
-
 ?

Subunits

Subunits Comment Organism
? x * 33654 + x * 44981, SDS-PAGE, MALDI-TOF and calculated Raphanus sativus

Synonyms

Synonyms Comment Organism
RsBGAL1
-
 Raphanus sativus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Raphanus sativus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 loss of 90% of activity Raphanus sativus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
28.6
-
 beta-(1-3)-galactobiose pH 4.0, 37°C Raphanus sativus
32.5
-
 beta-(1-3)-D-galactotriose pH 4.0, 37°C Raphanus sativus
123
-
 p-nitrophenyl-beta-galactoside pH 4.0, 37°C Raphanus sativus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4
-
 Raphanus sativus

pH Range

pH Minimum pH Maximum Comment Organism
2 6 almost inactive below and above Raphanus sativus