Crystallization (Comment) | Organism |
---|---|
wild type endoNF cocrystallized with oligomeric sialic acid and mutant enzymes H350A and R647A are crystallized by the hanging drop vapor diffusion method, using | Escherichia phage K1F |
Protein Variants | Comment | Organism |
---|---|---|
E581A | 1.8% relative activity compared to the wild type enzyme | Escherichia phage K1F |
H350A | 1.2% relative activity compared to the wild type enzyme | Escherichia phage K1F |
H350N | 3.4% relative activity compared to the wild type enzyme | Escherichia phage K1F |
H350Q | 4.4% relative activity compared to the wild type enzyme | Escherichia phage K1F |
H542A | 36% relative activity compared to the wild type enzyme | Escherichia phage K1F |
K410A | 20% relative activity compared to the wild type enzyme | Escherichia phage K1F |
R549A | 0.6% relative activity compared to the wild type enzyme | Escherichia phage K1F |
R647A | 0.17% relative activity compared to the wild type enzyme | Escherichia phage K1F |
W328R | 6.0% relative activity compared to the wild type enzyme | Escherichia phage K1F |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | Escherichia phage K1F | - |
fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | Q04830 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O | - |
Escherichia phage K1F | fragments of poly(sialic) acid or oligo(sialic) acids | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotrimer | x-ray crystallography | Escherichia phage K1F |
Synonyms | Comment | Organism |
---|---|---|
endoNF | - |
Escherichia phage K1F |
endosialidase | - |
Escherichia phage K1F |