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Literature summary for 3.2.1.129 extracted from

  • Schulz, E.C.; Schwarzer, D.; Frank, M.; Stummeyer, K.; Muehlenhoff, M.; Dickmanns, A.; Gerardy-Schahn, R.; Ficner, R.
    Structural basis for the recognition and cleavage of polysialic acid by the bacteriophage K1F tailspike protein endoNF (2010), J. Mol. Biol., 397, 341-351.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
wild type endoNF cocrystallized with oligomeric sialic acid and mutant enzymes H350A and R647A are crystallized by the hanging drop vapor diffusion method, using Escherichia phage K1F

Protein Variants

Protein Variants Comment Organism
E581A 1.8% relative activity compared to the wild type enzyme Escherichia phage K1F
H350A 1.2% relative activity compared to the wild type enzyme Escherichia phage K1F
H350N 3.4% relative activity compared to the wild type enzyme Escherichia phage K1F
H350Q 4.4% relative activity compared to the wild type enzyme Escherichia phage K1F
H542A 36% relative activity compared to the wild type enzyme Escherichia phage K1F
K410A 20% relative activity compared to the wild type enzyme Escherichia phage K1F
R549A 0.6% relative activity compared to the wild type enzyme Escherichia phage K1F
R647A 0.17% relative activity compared to the wild type enzyme Escherichia phage K1F
W328R 6.0% relative activity compared to the wild type enzyme Escherichia phage K1F

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O Escherichia phage K1F
-
fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F Q04830
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(sialic) acids or oligo(sialic) acids containing alpha-2,8-linked N-acetylneuraminic acid + H2O
-
Escherichia phage K1F fragments of poly(sialic) acid or oligo(sialic) acids
-
?

Subunits

Subunits Comment Organism
homotrimer x-ray crystallography Escherichia phage K1F

Synonyms

Synonyms Comment Organism
endoNF
-
Escherichia phage K1F
endosialidase
-
Escherichia phage K1F