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Literature summary for 3.2.1.129 extracted from

  • Murphy, J.A.; Hartwick, A.T.; Rutishauser, U.; Clarke, D.B.
    Endogenous polysialylated neural cell adhesion molecule enhances the survival of retinal ganglion cells (2009), Invest. Ophthalmol. Vis. Sci., 50, 861-869.
    View publication on PubMed

Application

Application Comment Organism
molecular biology removal of target molecules to investigate their function Escherichia phage K1F

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
polysialylated neural cell adhesion molecule + H2O Escherichia phage K1F polysialic acid is an unbranched homopolymer of 100-300 alpha-2,8-linked silica acid residues ?
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?

Organism

Organism UniProt Comment Textmining
Escherichia phage K1F
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Source Tissue

Source Tissue Comment Organism Textmining
additional information density of mouse retinal ganglion cells after removal of polysialic acid by endo-N treatment to investigate possible toxic effects of endo-N treatment Escherichia phage K1F
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additional information retinal ganglion cell cultures prepared from Long-Evans rat litters at postnatal day 7-8, and from 10-15 weeks old rats, for in vitro experiments Escherichia phage K1F
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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intravitreal endo-N injection and axotomy of optical nerve in wild-type mice: 4 days after treatment no difference in retinal ganglion cell density compared with untreated/uninjured, and vehicle treated/axotomy controls, at day 7 50% reduced density in vehicle treated/axotomy group compared to untreated/uninjured group, an additional 27% density reduction in endo-N treatment group Escherichia phage K1F
additional information
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intravitreal vehicle injection and axotomy of optical nerve in NCAM-/-mice: 4 days after treatment reduced density in vehicle injection/axotomy group compared to untreated/uninjured group, no neuroprotective effect of injection Escherichia phage K1F
additional information
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no differences in retinal ganglion cell densities after 14 days in NCAM-/-mice with or without endo-N treatment, shows non-toxicity of endo-N treatment itself Escherichia phage K1F
additional information
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polysialic acid is degraded from inner retina in vivo within 6 hours by intravitreal injection of 1 microl (6.7 U/microl) endo-N in 50% phosphate buffered saline with glycerol, from the outer retina within 24 hours, and remains absent at day 14l Escherichia phage K1F
additional information
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polysialic acid is degraded in vitro (neonatal cell culture) within 1 day by 1 microl and 3 microl endo-N, not by 0.5 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol Escherichia phage K1F
additional information
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polysialylated neural cell adhesion molecules are degraded in vitro (neonatal cell culture) within 12 hours after treatment with 1 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol, and remains absent 5 days after treatment, fewer retinal ganglion cells (54%) than control or vehicle control (96%) Escherichia phage K1F
additional information
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treatment with 1 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol, no significant difference in retinal ganglion cell densities 7 days after treatment, at day 14 reduction by approximately one third compared with control and vehicle control Escherichia phage K1F

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-2,8-linked polysialic acid + H2O
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Escherichia phage K1F ?
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?
polysialylated neural cell adhesion molecule + H2O polysialic acid is an unbranched homopolymer of 100-300 alpha-2,8-linked silica acid residues Escherichia phage K1F ?
-
?

Synonyms

Synonyms Comment Organism
endo-N
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Escherichia phage K1F
endoneuraminidase
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Escherichia phage K1F