Application | Comment | Organism |
---|---|---|
molecular biology | removal of target molecules to investigate their function | Escherichia phage K1F |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polysialylated neural cell adhesion molecule + H2O | Escherichia phage K1F | polysialic acid is an unbranched homopolymer of 100-300 alpha-2,8-linked silica acid residues | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage K1F | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | density of mouse retinal ganglion cells after removal of polysialic acid by endo-N treatment to investigate possible toxic effects of endo-N treatment | Escherichia phage K1F | - |
additional information | retinal ganglion cell cultures prepared from Long-Evans rat litters at postnatal day 7-8, and from 10-15 weeks old rats, for in vitro experiments | Escherichia phage K1F | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
intravitreal endo-N injection and axotomy of optical nerve in wild-type mice: 4 days after treatment no difference in retinal ganglion cell density compared with untreated/uninjured, and vehicle treated/axotomy controls, at day 7 50% reduced density in vehicle treated/axotomy group compared to untreated/uninjured group, an additional 27% density reduction in endo-N treatment group | Escherichia phage K1F |
additional information | - |
intravitreal vehicle injection and axotomy of optical nerve in NCAM-/-mice: 4 days after treatment reduced density in vehicle injection/axotomy group compared to untreated/uninjured group, no neuroprotective effect of injection | Escherichia phage K1F |
additional information | - |
no differences in retinal ganglion cell densities after 14 days in NCAM-/-mice with or without endo-N treatment, shows non-toxicity of endo-N treatment itself | Escherichia phage K1F |
additional information | - |
polysialic acid is degraded from inner retina in vivo within 6 hours by intravitreal injection of 1 microl (6.7 U/microl) endo-N in 50% phosphate buffered saline with glycerol, from the outer retina within 24 hours, and remains absent at day 14l | Escherichia phage K1F |
additional information | - |
polysialic acid is degraded in vitro (neonatal cell culture) within 1 day by 1 microl and 3 microl endo-N, not by 0.5 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol | Escherichia phage K1F |
additional information | - |
polysialylated neural cell adhesion molecules are degraded in vitro (neonatal cell culture) within 12 hours after treatment with 1 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol, and remains absent 5 days after treatment, fewer retinal ganglion cells (54%) than control or vehicle control (96%) | Escherichia phage K1F |
additional information | - |
treatment with 1 microl (6.7 U/microl) in 50% phosphate buffered saline with glycerol, no significant difference in retinal ganglion cell densities 7 days after treatment, at day 14 reduction by approximately one third compared with control and vehicle control | Escherichia phage K1F |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-2,8-linked polysialic acid + H2O | - |
Escherichia phage K1F | ? | - |
? | |
polysialylated neural cell adhesion molecule + H2O | polysialic acid is an unbranched homopolymer of 100-300 alpha-2,8-linked silica acid residues | Escherichia phage K1F | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-N | - |
Escherichia phage K1F |
endoneuraminidase | - |
Escherichia phage K1F |