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Literature summary for 3.2.1.117 extracted from

  • Zhou, J.; Hartmann, S.; Shepherd, B.K.; Poulton, J.E.
    Investigation of the microheterogeneity and aglycone specificity-conferring residues of black cherry prunasin hydrolases (2002), Plant Physiol., 129, 1252-1264.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.117

Cloned(Commentary)

Cloned (Comment) Organism
AH I expressed in Pichia pastoris Prunus serotina

Protein Variants

Protein Variants Comment Organism
G394D enhanced activity Prunus serotina
S216H enhanced activity Prunus serotina
S216H/G394D decreased activity Prunus serotina
S216H/Y220I enhanced activity Prunus serotina
S216H/Y220I/G394D decreased activity Prunus serotina
S220I/G394D decreased activity, EC 3.2.1.118 activity detected Prunus serotina
Y220I enhanced activity Prunus serotina

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-amygdalin + H2O Prunus serotina first step of cyanogenesis from amygdalin (R)-prunasin + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Prunus serotina Q945G7
-
-

Reaction

Reaction Comment Organism Reaction ID
(R)-amygdalin + H2O = (R)-prunasin + D-glucose high similarities with glycoside hydrolase family 1 enzymes Prunus serotina
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Prunus serotina
-
seed
-
 Prunus serotina
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-amygdalin + H2O
-
 Prunus serotina (R)-prunasin + D-glucose
-
 ?
(R)-amygdalin + H2O first step of cyanogenesis from amygdalin Prunus serotina (R)-prunasin + D-glucose
-
 ?