Cloned (Comment) | Organism |
---|---|
expression by an Aspergillus host | Aspergillus oryzae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxymannojirimycin | 0.2 mM, 50% inhibition | Aspergillus oryzae | |
EDTA | enzyme loses 96% activity after dialysis in 50 mM PIPES buffer, pH 6.0, containing 2 mM EDTA. Addition of 10 mM Ca2+ recovers activity to 100%, addition of 10 mM Mg2+ recovers activity to 43% | Aspergillus oryzae | |
kifunensine | 0.0015 mM, 50% inhibition | Aspergillus oryzae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 | Aspergillus oryzae | - |
- |
intracellular | with strain 22A1, the ratio of intracellular to extracellular 1,2-alpha-mannosidase activity is about 4:1 | Aspergillus oryzae | 5622 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM, completely recovers activity after treatment with EDTA | Aspergillus oryzae | |
Mg2+ | 10 mM, recovers activity to 43% after treatment with EDTA | Aspergillus oryzae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
x * 67000, SDS-PAGE | Aspergillus oryzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus oryzae | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Aspergillus oryzae |
Purification (Comment) | Organism |
---|---|
- |
Aspergillus oryzae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture supernatant | - |
Aspergillus oryzae | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Aspergillus oryzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-alpha-D-mannobiose + H2O | - |
Aspergillus oryzae | D-mannose | - |
? | |
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Aspergillus oryzae | (Man)5GlcNAc + 4 D-mannose | - |
? | |
additional information | no activity with p-nitrophenyl-alpha-mannoside, Manalpha(1-3)Man-OMe or Manalpha(1-6)Man-OMe | Aspergillus oryzae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Aspergillus oryzae |
Synonyms | Comment | Organism |
---|---|---|
FmanIBp | - |
Aspergillus oryzae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 60 | 45°C: optimum, 60°C: 60% of maximal activity, 65°C: 14% of maximal activity, hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
hydrolysis of 1,2-alpha-mannobiose | Aspergillus oryzae |