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Literature summary for 3.2.1.113 extracted from
- Isolation and purification of a neutral alpha(1,2)-mannosidase from Trypanosoma cruzi (1999), Glycobiology, 9, 423-433.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1-deoxymannojirimycin | not significantly | Trypanosoma cruzi |  |
| D-mannono-delta-lactam | - |
Trypanosoma cruzi | |
| D-mannono-gamma-lactone | - |
Trypanosoma cruzi | |
| EDTA | strong | Trypanosoma cruzi | |
| Fe2+ | strong | Trypanosoma cruzi | |
| kifunensine | - |
Trypanosoma cruzi | |
| swainsonine | - |
Trypanosoma cruzi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.087 | - |
p-nitrophenyl-alpha-D-mannoside | 37°C, pH 6.1 | Trypanosoma cruzi | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Trypanosoma cruzi | |
| Ni2+ | activation | Trypanosoma cruzi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
2 * 76000, SDS-PAGE | Trypanosoma cruzi |
| 150000 | 160000 | gel filtration | Trypanosoma cruzi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma cruzi | - |
intracellular protozoan, causes Chagas disease in humans, epimastigote stage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O | - |
Trypanosoma cruzi | (Man)5GlcNAc + 4 D-mannose | product is Man6GlcNAc | ? | |
| additional information | no hydrolysis of alpha1,3-linkages | Trypanosoma cruzi | ? | - |
? | |
| additional information | no hydrolysis of alpha1,6-linkages | Trypanosoma cruzi | ? | - |
? | |
| p-nitrophenyl-alpha-D-mannoside + H2O | - |
Trypanosoma cruzi | 4-nitrophenol + D-mannose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 76000, SDS-PAGE | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.9 | 6.5 | - |
Trypanosoma cruzi |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0001 | - |
swainsonine | pH 6.1, 37°C | Trypanosoma cruzi | |
| 0.002 | - |
D-mannono-delta-lactam | pH 6.1, 37°C | Trypanosoma cruzi | |
| 0.006 | - |
kifunensine | pH 6.1, 37°C | Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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