Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.113 extracted from

  • Forsee, W.T.; Springfield, J.D.; Schutzbach, J.S.
    Effect of phospholipids on alpha-1,2-mannosidase activity (1982), J. Biol. Chem., 257, 9963-9967.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information no activation by negatively charged phospholipids, dibutyryl-phosphocholine Oryctolagus cuniculus
phosphatidylcholine activation, with acyl-chains of different lengths, above C-4 Oryctolagus cuniculus
phosphatidylethanolamine activation, in the presence of 3.5 mM Triton X-100 Oryctolagus cuniculus
Triton X-100 activation together with zwitterionic phospholipids Oryctolagus cuniculus
Zwitterionic phospholipids activation, required for solubilized and purified enzyme, independent of acyl chain length or degree of saturation, an ordered lipid structure of either micelles or bilayers, mixed micelles together with Triton X-100 Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Oryctolagus cuniculus 16020
-
microsome
-
Oryctolagus cuniculus
-
-

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, solubilization requires non-ionic detergents Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc Oryctolagus cuniculus (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 6.5 in the presence of egg yolk lyso-phosphatidylcholine Oryctolagus cuniculus
6.5
-
in the presence of soybean phosphatidylinositol Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
5.2 7.3 about half-maximal activity at pH 5.2 and 7.3, in the presence of egg yolk lyso-phosphatidylcholine Oryctolagus cuniculus
5.5 7.5 about half-maximal activity at pH 5.5 and 7.5, in the presence of soybean phosphatidylinositol Oryctolagus cuniculus