Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activation by negatively charged phospholipids, dibutyryl-phosphocholine | Oryctolagus cuniculus | |
phosphatidylcholine | activation, with acyl-chains of different lengths, above C-4 | Oryctolagus cuniculus | |
phosphatidylethanolamine | activation, in the presence of 3.5 mM Triton X-100 | Oryctolagus cuniculus | |
Triton X-100 | activation together with zwitterionic phospholipids | Oryctolagus cuniculus | |
Zwitterionic phospholipids | activation, required for solubilized and purified enzyme, independent of acyl chain length or degree of saturation, an ordered lipid structure of either micelles or bilayers, mixed micelles together with Triton X-100 | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Oryctolagus cuniculus | 16020 | - |
microsome | - |
Oryctolagus cuniculus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, solubilization requires non-ionic detergents | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | i.e. Manalpha(1-2)Manalpha(1-2)Manalpha(1-3) (Manalpha(1-6))ManbetaGlcNAc-betaGlcNAc | Oryctolagus cuniculus | (Man)4(GlcNAc)2 + (Man)3(GlcNAc)2 + D-mannose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 6.5 | in the presence of egg yolk lyso-phosphatidylcholine | Oryctolagus cuniculus |
6.5 | - |
in the presence of soybean phosphatidylinositol | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.2 | 7.3 | about half-maximal activity at pH 5.2 and 7.3, in the presence of egg yolk lyso-phosphatidylcholine | Oryctolagus cuniculus |
5.5 | 7.5 | about half-maximal activity at pH 5.5 and 7.5, in the presence of soybean phosphatidylinositol | Oryctolagus cuniculus |