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Literature summary for 3.2.1.113 extracted from

  • Szumilo, T.; Kaushal, G.P.; Hori, H.; Elbein, A.D.
    Purification and properties of a glycoprotein processing alpha-mannosidase from mung bean seedlings (1986), Plant Physiol., 81, 383-389.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.113

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activation Vigna radiata var. radiata
Triton X-100 0.1% Vigna radiata var. radiata

Inhibitors

Inhibitors Comment Organism Structure
1,4-Dideoxy-1,4-imino-D-mannitol not Vigna radiata var. radiata
1-deoxymannojirimycin
-
 Vigna radiata var. radiata
alpha1,2-Linked oligosaccharides
-
 Vigna radiata var. radiata
alpha1,3-Linked oligosaccharides
-
 Vigna radiata var. radiata
EDTA Ca2+ restores Vigna radiata var. radiata
mannosamine
-
 Vigna radiata var. radiata
additional information no inhibition by mannose, alpha1,6-linked mannose oligosaccharide, castanospermine, deoxynojirimycin, 2,5-dihydroxymethyl-3,4-dihydroxypyrrolidine Vigna radiata var. radiata

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Vigna radiata var. radiata 16020
-
microsome
-
 Vigna radiata var. radiata
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activation Vigna radiata var. radiata
Co2+ slight activation Vigna radiata var. radiata
Cu2+ slight activation Vigna radiata var. radiata
Fe2+ slight activation Vigna radiata var. radiata
Mg2+ not Vigna radiata var. radiata
Mn2+ slight activation Vigna radiata var. radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O Vigna radiata var. radiata
-
 (Man)5GlcNAc + 4 D-mannose
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on mannan and mannosamine-Sepharose Vigna radiata var. radiata

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Vigna radiata var. radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vigna radiata var. radiata

Storage Stability

Storage Stability Organism
0-4°C, in buffer with 10% glycerol, purified enzyme, a few days, partially purified enzyme, at least 2 weeks Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Vigna radiata var. radiata (Man)5GlcNAc + 4 D-mannose
-
 ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Vigna radiata var. radiata (Man)5GlcNAc + 4 D-mannose after long-term incubation ?
Manalpha(1-2)Manalpha(1-6)(Manalpha(1-2)Manalpha(1-3))Manalpha(1-6)Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)Manbeta(1-4)GlcNAc + 4 H2O
-
 Vigna radiata var. radiata (Man)5GlcNAc + 4 D-mannose (Man)7GlcNAc and (Man)6GlcNAc after 4 h, (Man)8GlcNAc, (Man)7GlcNAc, (Man)6GlcNAc and (Man)5GlcNAc after 12 h incubation ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
4.8 6.8 about half-maximal activity at pH 4.8 and 6.8 Vigna radiata var. radiata