Protein Variants | Comment | Organism |
---|---|---|
I34H | mutation to corresponding residue of Bacillus licheniformis, complete loss of catalytic activity | Bacillus amyloliquefaciens |
P407H | mutation to corresponding residue of Bacillus licheniformis, leads to increase in thermostability without significant changes in kinetic parameters. Mutant displays a more rigid structure than wild-type | Bacillus amyloliquefaciens |
Q67H | mutation to corresponding residue of Bacillus licheniformis, leads to increase in thermostability without significant changes in kinetic parameters. Flexibility of mutant is increased compared to wild-type | Bacillus amyloliquefaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Bacillus amyloliquefaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus amyloliquefaciens ATCC 23350 | - |
- |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Bacillus amyloliquefaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
wild-type, 75% of initial activity after 80 min, mutant Q67H after 185 min, and mutant P407H 125 min of incubation, respectively | Bacillus amyloliquefaciens |
75 | - |
wild-type, half-life 125 min, mutant Q67H, 145 min, and mutant P407H, 185 min | Bacillus amyloliquefaciens |