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Literature summary for 3.2.1.1 extracted from
- Biochemical and structural characterization of Amy1: an alpha-amylase from Cryptococcus flavus expressed in Saccharomyces cerevisiae (2011), Enzyme Res., 2011, 157294.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Sacharomyces cerevisiae strain CENPK2 | Saitozyma flava |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | complete inhibition at 4 mM | Saitozyma flava |  |
| Hg2+ | complete inhibition at 4 mM | Saitozyma flava | |
| Mg2+ | 11% inhibition at 4 mM | Saitozyma flava | |
| Mn2+ | 22% inhibition at 4 mM | Saitozyma flava | |
| Zn2+ | 80% inhibition at 4 mM | Saitozyma flava | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saitozyma flava | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | activates | Saitozyma flava | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
x * 67000, SDS-PAGE | Saitozyma flava |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saitozyma flava | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 3.8fold from Saccharomyces cerevisiae by anion exchange chromatography and gel filtration to homogeneity | Saitozyma flava |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.16 | - |
purified recombinant enzyme, pH 5.5, 60°C | Saitozyma flava |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Saitozyma flava | ? | - |
? | |
| amylose + H2O | - |
Saitozyma flava | ? | - |
? | |
| glycogen + H2O | - |
Saitozyma flava | ? | - |
? | |
| additional information | activity of Amy1 towards soluble starch, glycogen, amylopectin, amylose in descending order | Saitozyma flava | ? | - |
? | |
| soluble starch + H2O | - |
Saitozyma flava | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 67000, SDS-PAGE | Saitozyma flava |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AMY1 | - |
Saitozyma flava |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Saitozyma flava |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | activity range, profile, overview | Saitozyma flava |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
purified recombinant enzyme, 1 h, 90% activity remaining | Saitozyma flava |
| 60 | - |
purified recombinant enzyme, 1 h, 50% activity remaining | Saitozyma flava |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Saitozyma flava |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 8 | activity range, profile, overview | Saitozyma flava |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | over 80% acivity remaining within this range | Saitozyma flava |
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