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Literature summary for 3.2.1.1 extracted from
- Kinetic analysis and mechanism on the inhibition of chlorogenic acid and its components against porcine pancreas alpha-amylase isozymes I and II (2009), J. Agric. Food Chem., 57, 9218-9225.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| caffeic acid | mixed-type inhibition, almost all activities of isozymes PPA-I and PPA-II are lost in the presence of 4 mM | Sus scrofa |  |
| chlorogenic acid | 5-caffeoylquinic acid, mixed-type inhibition, complete inhibition of isozymes PPA-I is observed at 1.5 mM and that of isozyme PPA-II is at 2.0 mM | Sus scrofa | |
| quinic acid | poor inhibitor, activities higher than 80% remain for both isozymes even in the presence of 15 mM quinic acid, while they decrease sharply when the quinic acid concentration increases from 15 to 35 mM. Complete inhibition of isozyme PPAI is given with 35.0 mM, and that of isozyme PPA-II is with 40 mM quinic acid | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.48 | - |
4-nitrophenyl-alpha-D-maltoside | isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | |
| 4.63 | - |
4-nitrophenyl-alpha-D-maltoside | isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55400 | - |
SDS-PAGE | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TSKgel BioAssist Q column chromatography, gel filtration | Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-D-maltoside + H2O | - |
Sus scrofa | 4-nitrophenol + maltose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPA-I | isozyme | Sus scrofa |
| PPA-II | isozyme | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0494 | - |
4-nitrophenyl-alpha-D-maltoside | isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | |
| 0.0497 | - |
4-nitrophenyl-alpha-D-maltoside | isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
chlorogenic acid | isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | |
| 0.23 | - |
chlorogenic acid | isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | |
| 1.12 | - |
caffeic acid | isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | |
| 1.64 | - |
caffeic acid | isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | chlorogenic acid | |
| 0.08 | - |
isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | chlorogenic acid | |
| 0.37 | - |
isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | caffeic acid | |
| 0.4 | - |
isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | caffeic acid | |
| 25.3 | - |
isozyme PPA-II, at pH 6.9 and 30°C | Sus scrofa | quinic acid | |
| 26.5 | - |
isozyme PPA-I, at pH 6.9 and 30°C | Sus scrofa | quinic acid | |
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