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Literature summary for 3.2.1.1 extracted from

  • Mollania, N.; Khajeh, K.; Hosseinkhani, S.; Dabirmanesh, B.
    Purification and characterization of a thermostable phytate resistant alpha-amylase from Geobacillus sp. LH8 (2010), Int. J. Biol. Macromol., 46, 27-36.
    View publication on PubMed
Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Geobacillus sp.

General Stability

General Stability Organism
Ca2+ strongly stabilizes alpha-amylase while EDTA reduces its stability Geobacillus sp.

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 80% residual activity at 10 mM Geobacillus sp.
Co2+ 70% residual activity at 10 mM Geobacillus sp.
Cu2+ 86% residual activity at 10 mM Geobacillus sp.
EDTA 21% residual activity at 10 mM, alpha-amylase is completely inactivated after 6 min of incubation at 80°C in the presence of 5 mM EDTA Geobacillus sp.
Fe3+ 10% residual activity at 10 mM Geobacillus sp.
Mg2+ 40% residual activity at 10 mM Geobacillus sp.
additional information not inhibited by phytic acid Geobacillus sp.
Ni2+ 60% residual activity at 10 mM Geobacillus sp.
Zn2+ 64% residual activity at 10 mM Geobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the Km value for starch is 3 mg/ml at 65°C using potato starch (1% (w/v)) as a substrate in 20 mM Tris-HCl, pH 7.4 Geobacillus sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ 120% relative activity at 10 mM Geobacillus sp.
Ca2+ 200% relative activity at 10 mM Geobacillus sp.
Cr3+ 140% relative activity at 10 mM Geobacillus sp.
K+ 105% relative activity at 10 mM Geobacillus sp.
Mn2+ 140% relative activity at 10 mM Geobacillus sp.
additional information not stimulated by Na+ Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 SDS-PAGE Geobacillus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
-
-
Geobacillus sp. LH8
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Q-Sepharose column chromatography, and Mono Q-Sepharose column chromatography Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.6
-
 after 13.6fold purification, at 65°C Geobacillus sp.
59
-
 crude extract, at 65°C Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
potato starch + H2O
-
 Geobacillus sp. malto-oligosaccharides
-
 ?
potato starch + H2O
-
 Geobacillus sp. LH8 malto-oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
1,4-alpha-D-glucan glucanohydrolase
-
 Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 in the absence of Ca2+ Geobacillus sp.
80
-
 in the presence of 10 mM Ca2+ Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85 90 29% activity is seen after 10 min at 85 and 90°C, respectively Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7
-
 Geobacillus sp.