Protein Variants | Comment | Organism |
---|---|---|
N172R/H156Y/A181T | the mutations increase the thermostability of alpha-amylase by 5fold | Bacillus licheniformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Bacillus subtilis alpha-amylase requires roughly 4times more calcium for full activity than other alpha-amylases of nonbacterial nature | Bacillus subtilis | |
additional information | does not require Ca2+ | Pyrococcus furiosus | |
additional information | does not require Ca2+ | Pyrococcus woesei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
- |
Bacillus licheniformis |
58000 | - |
SDS-PAGE | Bacillus amyloliquefaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | Lactiplantibacillus plantarum | - |
? | - |
? | |
glycogen + H2O | Bacillus subtilis | - |
? | - |
? | |
glycogen + H2O | Geobacillus stearothermophilus | - |
? | - |
? | |
glycogen + H2O | Pyrococcus furiosus | - |
? | - |
? | |
glycogen + H2O | Bacillus licheniformis | - |
? | - |
? | |
glycogen + H2O | Bacillus amyloliquefaciens | - |
? | - |
? | |
glycogen + H2O | Rhodothermus marinus | - |
? | - |
? | |
glycogen + H2O | Pyrococcus woesei | - |
? | - |
? | |
glycogen + H2O | Thermotoga maritima | - |
? | - |
? | |
glycogen + H2O | Lederbergia lentus | - |
? | - |
? | |
glycogen + H2O | Thermoactinomyces vulgaris | - |
? | - |
? | |
glycogen + H2O | Rhizopus sp. | - |
? | - |
? | |
glycogen + H2O | Thermomyces lanuginosus | - |
? | - |
? | |
glycogen + H2O | Thermococcus litoralis | - |
? | - |
? | |
glycogen + H2O | Thermothelomyces heterothallicus | - |
? | - |
? | |
glycogen + H2O | Chloroflexus aurantiacus | - |
? | - |
? | |
glycogen + H2O | Thermus filiformis | - |
? | - |
? | |
glycogen + H2O | Mycothermus thermophilus | - |
? | - |
? | |
glycogen + H2O | Lipomyces kononenkoae | - |
? | - |
? | |
glycogen + H2O | Alicyclobacillus acidocaldarius | - |
? | - |
? | |
glycogen + H2O | Dictyoglomus thermophilum | - |
? | - |
? | |
glycogen + H2O | Thermococcus hydrothermalis | - |
? | - |
? | |
glycogen + H2O | Desulfurococcus mucosus | - |
? | - |
? | |
glycogen + H2O | Anoxybacillus flavithermus | - |
? | - |
? | |
glycogen + H2O | Thermococcus profundus | - |
? | - |
? | |
glycogen + H2O | Pyrodictium abyssi | - |
? | - |
? | |
glycogen + H2O | Thermococcus fumicolans | - |
? | - |
? | |
glycogen + H2O | Staphylothermus marinus | - |
? | - |
? | |
glycogen + H2O | Lactobacillus amylovorus | - |
? | - |
? | |
glycogen + H2O | Halothermothrix orenii | - |
? | - |
? | |
glycogen + H2O | Thermococcus aggregans | - |
? | - |
? | |
glycogen + H2O | Thermococcus celer | - |
? | - |
? | |
glycogen + H2O | Thermococcus guaymasensis | - |
? | - |
? | |
starch + H2O | Lactiplantibacillus plantarum | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Bacillus subtilis | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Geobacillus stearothermophilus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Pyrococcus furiosus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Bacillus licheniformis | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Bacillus amyloliquefaciens | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Rhodothermus marinus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Pyrococcus woesei | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermotoga maritima | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Lederbergia lentus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermoactinomyces vulgaris | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Rhizopus sp. | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermomyces lanuginosus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus litoralis | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermothelomyces heterothallicus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Chloroflexus aurantiacus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermus filiformis | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Mycothermus thermophilus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Lipomyces kononenkoae | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Alicyclobacillus acidocaldarius | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Dictyoglomus thermophilum | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus hydrothermalis | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Desulfurococcus mucosus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Anoxybacillus flavithermus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus profundus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Pyrodictium abyssi | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus fumicolans | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Staphylothermus marinus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Lactobacillus amylovorus | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Halothermothrix orenii | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus aggregans | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus celer | - |
malto-oligosaccharides | - |
? | |
starch + H2O | Thermococcus guaymasensis | - |
malto-oligosaccharides | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alicyclobacillus acidocaldarius | - |
- |
- |
Anoxybacillus flavithermus | - |
- |
- |
Bacillus amyloliquefaciens | - |
- |
- |
Bacillus licheniformis | - |
- |
- |
Bacillus subtilis | - |
- |
- |
Chloroflexus aurantiacus | - |
- |
- |
Desulfurococcus mucosus | - |
- |
- |
Dictyoglomus thermophilum | - |
- |
- |
Geobacillus stearothermophilus | - |
- |
- |
Halothermothrix orenii | - |
- |
- |
Lactiplantibacillus plantarum | - |
- |
- |
Lactobacillus amylovorus | - |
- |
- |
Lederbergia lentus | - |
- |
- |
Lipomyces kononenkoae | - |
- |
- |
Mycothermus thermophilus | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Pyrococcus woesei | - |
- |
- |
Pyrodictium abyssi | - |
- |
- |
Rhizopus sp. | - |
- |
- |
Rhodothermus marinus | - |
- |
- |
Staphylothermus marinus | - |
- |
- |
Thermoactinomyces vulgaris | - |
- |
- |
Thermococcus aggregans | - |
- |
- |
Thermococcus celer | - |
- |
- |
Thermococcus fumicolans | - |
- |
- |
Thermococcus guaymasensis | - |
- |
- |
Thermococcus hydrothermalis | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Thermococcus profundus | - |
- |
- |
Thermomyces lanuginosus | - |
- |
- |
Thermothelomyces heterothallicus | - |
- |
- |
Thermotoga maritima | - |
- |
- |
Thermus filiformis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Lactiplantibacillus plantarum | ? | - |
? | |
glycogen + H2O | - |
Bacillus subtilis | ? | - |
? | |
glycogen + H2O | - |
Geobacillus stearothermophilus | ? | - |
? | |
glycogen + H2O | - |
Pyrococcus furiosus | ? | - |
? | |
glycogen + H2O | - |
Bacillus licheniformis | ? | - |
? | |
glycogen + H2O | - |
Bacillus amyloliquefaciens | ? | - |
? | |
glycogen + H2O | - |
Rhodothermus marinus | ? | - |
? | |
glycogen + H2O | - |
Pyrococcus woesei | ? | - |
? | |
glycogen + H2O | - |
Thermotoga maritima | ? | - |
? | |
glycogen + H2O | - |
Lederbergia lentus | ? | - |
? | |
glycogen + H2O | - |
Thermoactinomyces vulgaris | ? | - |
? | |
glycogen + H2O | - |
Rhizopus sp. | ? | - |
? | |
glycogen + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
glycogen + H2O | - |
Thermococcus litoralis | ? | - |
? | |
glycogen + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? | |
glycogen + H2O | - |
Chloroflexus aurantiacus | ? | - |
? | |
glycogen + H2O | - |
Thermus filiformis | ? | - |
? | |
glycogen + H2O | - |
Mycothermus thermophilus | ? | - |
? | |
glycogen + H2O | - |
Lipomyces kononenkoae | ? | - |
? | |
glycogen + H2O | - |
Alicyclobacillus acidocaldarius | ? | - |
? | |
glycogen + H2O | - |
Dictyoglomus thermophilum | ? | - |
? | |
glycogen + H2O | - |
Thermococcus hydrothermalis | ? | - |
? | |
glycogen + H2O | - |
Desulfurococcus mucosus | ? | - |
? | |
glycogen + H2O | - |
Anoxybacillus flavithermus | ? | - |
? | |
glycogen + H2O | - |
Thermococcus profundus | ? | - |
? | |
glycogen + H2O | - |
Pyrodictium abyssi | ? | - |
? | |
glycogen + H2O | - |
Thermococcus fumicolans | ? | - |
? | |
glycogen + H2O | - |
Staphylothermus marinus | ? | - |
? | |
glycogen + H2O | - |
Lactobacillus amylovorus | ? | - |
? | |
glycogen + H2O | - |
Halothermothrix orenii | ? | - |
? | |
glycogen + H2O | - |
Thermococcus aggregans | ? | - |
? | |
glycogen + H2O | - |
Thermococcus celer | ? | - |
? | |
glycogen + H2O | - |
Thermococcus guaymasensis | ? | - |
? | |
starch + H2O | - |
Lactiplantibacillus plantarum | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Bacillus subtilis | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Geobacillus stearothermophilus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Pyrococcus furiosus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Bacillus licheniformis | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Bacillus amyloliquefaciens | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Rhodothermus marinus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Pyrococcus woesei | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermotoga maritima | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Lederbergia lentus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermoactinomyces vulgaris | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Rhizopus sp. | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermomyces lanuginosus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus litoralis | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermothelomyces heterothallicus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Chloroflexus aurantiacus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermus filiformis | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Mycothermus thermophilus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Lipomyces kononenkoae | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Alicyclobacillus acidocaldarius | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Dictyoglomus thermophilum | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus hydrothermalis | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Desulfurococcus mucosus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Anoxybacillus flavithermus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus profundus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Pyrodictium abyssi | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus fumicolans | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Staphylothermus marinus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Lactobacillus amylovorus | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Halothermothrix orenii | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus aggregans | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus celer | malto-oligosaccharides | - |
? | |
starch + H2O | - |
Thermococcus guaymasensis | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 58000 | Bacillus licheniformis |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,4-glucan-4-glucanohydrolase | - |
Lactiplantibacillus plantarum |
alpha-1,4-glucan-4-glucanohydrolase | - |
Bacillus subtilis |
alpha-1,4-glucan-4-glucanohydrolase | - |
Geobacillus stearothermophilus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Pyrococcus furiosus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Bacillus licheniformis |
alpha-1,4-glucan-4-glucanohydrolase | - |
Bacillus amyloliquefaciens |
alpha-1,4-glucan-4-glucanohydrolase | - |
Rhodothermus marinus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Pyrococcus woesei |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermotoga maritima |
alpha-1,4-glucan-4-glucanohydrolase | - |
Lederbergia lentus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermoactinomyces vulgaris |
alpha-1,4-glucan-4-glucanohydrolase | - |
Rhizopus sp. |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermomyces lanuginosus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus litoralis |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermothelomyces heterothallicus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Chloroflexus aurantiacus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermus filiformis |
alpha-1,4-glucan-4-glucanohydrolase | - |
Mycothermus thermophilus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Lipomyces kononenkoae |
alpha-1,4-glucan-4-glucanohydrolase | - |
Alicyclobacillus acidocaldarius |
alpha-1,4-glucan-4-glucanohydrolase | - |
Dictyoglomus thermophilum |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus hydrothermalis |
alpha-1,4-glucan-4-glucanohydrolase | - |
Desulfurococcus mucosus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Anoxybacillus flavithermus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus profundus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Pyrodictium abyssi |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus fumicolans |
alpha-1,4-glucan-4-glucanohydrolase | - |
Staphylothermus marinus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Lactobacillus amylovorus |
alpha-1,4-glucan-4-glucanohydrolase | - |
Halothermothrix orenii |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus aggregans |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus celer |
alpha-1,4-glucan-4-glucanohydrolase | - |
Thermococcus guaymasensis |
Amy | - |
Alicyclobacillus acidocaldarius |
Amy | - |
Anoxybacillus flavithermus |
Amy B | - |
Halothermothrix orenii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermomyces lanuginosus |
60 | - |
- |
Mycothermus thermophilus |
60 | - |
- |
Anoxybacillus flavithermus |
60 | - |
- |
Halothermothrix orenii |
60 | 65 | - |
Rhizopus sp. |
60 | 65 | - |
Lactobacillus amylovorus |
61 | - |
- |
Rhodothermus marinus |
62.5 | - |
- |
Thermoactinomyces vulgaris |
65 | - |
- |
Lactiplantibacillus plantarum |
65 | - |
- |
Staphylothermus marinus |
70 | - |
- |
Bacillus subtilis |
70 | - |
- |
Bacillus amyloliquefaciens |
70 | - |
- |
Lederbergia lentus |
70 | - |
- |
Lipomyces kononenkoae |
70 | 80 | - |
Geobacillus stearothermophilus |
71 | - |
- |
Chloroflexus aurantiacus |
75 | - |
- |
Alicyclobacillus acidocaldarius |
80 | - |
- |
Thermococcus profundus |
85 | - |
- |
Thermococcus hydrothermalis |
85 | 90 | - |
Thermotoga maritima |
88 | - |
- |
Thermococcus litoralis |
90 | - |
- |
Dictyoglomus thermophilum |
90 | - |
- |
Thermococcus celer |
95 | - |
- |
Thermus filiformis |
95 | - |
- |
Thermococcus fumicolans |
100 | - |
- |
Pyrococcus furiosus |
100 | - |
- |
Bacillus licheniformis |
100 | - |
- |
Pyrococcus woesei |
100 | - |
- |
Thermothelomyces heterothallicus |
100 | - |
- |
Desulfurococcus mucosus |
100 | - |
- |
Pyrodictium abyssi |
100 | - |
- |
Thermococcus aggregans |
100 | - |
- |
Thermococcus guaymasensis |