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Literature summary for 3.2.1.1 extracted from

  • Tarling, C.A.; Woods, K.; Zhang, R.; Brastianos, H.C.; Brayer, G.D.; Andersen, R.J.; Withers, S.G.
    The search for novel human pancreatic alpha-amylase inhibitors: high-throughput screening of terrestrial and marine natural product extracts (2008), ChemBioChem, 9, 433-438.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ethyl caffeate pH 7.0 Homo sapiens
montbretin A glycosylated acyl-flavonols, originally isolated from an extract of Crocosmia crocosmiiflora, measured in the presence and in the absence of 5 mM dithiothreitol, competitive inhibitor, more effective than montbretin B and C due to its free meta-hydroxyl group of the cinnamic acid moiety Homo sapiens
montbretin B glycosylated acyl-flavonols, originally isolated from an extract of Crocosmia crocosmiiflora, less effective than montbretin A due to the hydroxy group of montbretin A in the cinnamic acid moiety, respnsible for the tight binding Homo sapiens
montbretin C glycosylated acyl-flavonols, a methyl ether of the cinnamic acid moiety, originally isolated from an extract of Crocosmia crocosmiiflora, less effective than montbretin A due to the hydroxy group of montbretin A in the cinnamic acid moiety, respnsible for the tight binding Homo sapiens
additional information no effect by the addtion of 0.1% dimethyl sulfoxide and 0.01% Triton X-100 Homo sapiens
myricetin pH 7.0 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
2-chloro-4-nitrophenyl-alpha-D-maltotrioside commercial substrate, pH 7.0 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl-alpha-D-maltotrioside + H2O commercial substrate for a flourescence-based assay, pH 7.0 Homo sapiens 2-chloro-4-nitrophenol + alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000081
-
montbretin A pH 7.0, in presence of 1 mM CaCl2 Homo sapiens
0.0013
-
ethyl caffeate pH 7.0, uncompetitive inhibition Homo sapiens
0.0036
-
montbretin B pH 7.0, in presence of 1 mM CaCl2 Homo sapiens
0.0061
-
montbretin C pH 7.0, in presence of 1 mM CaCl2 Homo sapiens
0.11
-
myricetin pH 7.0, competitive inhibition Homo sapiens