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Literature summary for 3.2.1.1 extracted from

  • Maurus, R.; Begum, A.; Williams, L.K.; Fredriksen, J.R.; Zhang, R.; Withers, S.G.; Brayer, G.D.
    Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity (2008), Biochemistry, 47, 3332-3344.
    View publication on PubMed
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
Br- greater affinity for the enzyme than chloride and a comparable relative activity Homo sapiens
Cl- allosteric activation. The enzyme is essentially inactive in the absence of chloride ion Homo sapiens
ClO3- activates, optimal concentration is 0.2 mM Homo sapiens
HCOO- activates, optimal concentration is 1 mM Homo sapiens
I- lower affinity for the enzyme than chloride and higher relative activity Homo sapiens
N3- activates, optimal concentration is 1 mM Homo sapiens
NO2- activates, optimal concentration is 1 mM Homo sapiens
NO3- activates, optimal concentration is 0.1 mM Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant wild-type and N298S variant HPAs are crystallized using the hanging drop vapor diffusion method Homo sapiens

Protein Variants

Protein Variants Comment Organism
N298S variant of the enzyme has an approximate 200fold reduction in affinity for chloride ion Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.1 mM NO3- Homo sapiens
0.57
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
0.58
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.2 mM ClO3- Homo sapiens
0.58
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM NO3- Homo sapiens
0.69
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
0.76
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Cl- Homo sapiens
1.2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Br- Homo sapiens
1.3
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM I- Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P04746
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl alpha-maltotrioside + H2O
-
 Homo sapiens ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Br- Homo sapiens
2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.2 mM ClO3- Homo sapiens
2.1
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM I- Homo sapiens
2.2
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.1 mM NO3- Homo sapiens
2.4
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 0.01 mM Cl- Homo sapiens
3.5
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM NO2- Homo sapiens
4.4
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens
15.8
-
 2-chloro-4-nitrophenyl alpha-maltotrioside in presence of 1 mM N3- Homo sapiens