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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of an extracellular alpha-amylase from Bacillus subtilis AX20 (2005), Protein Expr. Purif., 41, 349-354.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Bacillus subtilis |  |
| Cu2+ | - |
Bacillus subtilis | |
| Hg2+ | - |
Bacillus subtilis | |
| additional information | mo inhibition by EDTA or EGTA | Bacillus subtilis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme shows no requirement for metals | Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
2 * 79000, SDS-PAGE | Bacillus subtilis |
| 139400 | - |
gel filtration | Bacillus subtilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Bacillus subtilis | - |
maltose + D-glucose | - |
? | |
| starch + H2O | Bacillus subtilis AX20 | - |
maltose + D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isolated from soil | - |
| Bacillus subtilis AX20 | - |
isolated from soil | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bacillus subtilis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 37.5fold to homogeneity by ammonium sulfate fractionation, ion exchange and starch affinity chromatography, and gel filtration | Bacillus subtilis |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| (alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | endo mode of action | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4133 | - |
purified enzyme | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | - |
Bacillus subtilis | maltose + D-glucose | - |
? | |
| starch + H2O | raw starch from corn and soluble starch, endo mode of action | Bacillus subtilis | maltose + D-glucose | 25% maltose, 75% glucose | ? | |
| starch + H2O | - |
Bacillus subtilis AX20 | maltose + D-glucose | - |
? | |
| starch + H2O | raw starch from corn and soluble starch, endo mode of action | Bacillus subtilis AX20 | maltose + D-glucose | 25% maltose, 75% glucose | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 79000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
30 min, purified enzyme, stable | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus subtilis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | purified enzyme, stable, 24 h at 4°C | Bacillus subtilis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bacillus subtilis | isoelectric focusing | - |
7 |
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