Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition by phytate at up to 10 mM | Geobacillus thermodenitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.05 | - |
soluble starch | pH 5.5, 80°C | Geobacillus thermodenitrificans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Geobacillus thermodenitrificans | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stabilizes the enzyme | Geobacillus thermodenitrificans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Geobacillus thermodenitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermodenitrificans | - |
- |
- |
Geobacillus thermodenitrificans HRO10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 13.6fold to homogeneity in a multistep procedure | Geobacillus thermodenitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble starch + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
soluble starch + H2O | - |
Geobacillus thermodenitrificans HRO10 | ? | - |
? | |
starch + H2O | soluble starch from potato | Geobacillus thermodenitrificans | malto-oligosaccharides | - |
? | |
starch + H2O | soluble starch from potato | Geobacillus thermodenitrificans HRO10 | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Geobacillus thermodenitrificans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Geobacillus thermodenitrificans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, in presence of Ca2+, 92% remaining activity | Geobacillus thermodenitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Geobacillus thermodenitrificans |