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Literature summary for 3.2.1.1 extracted from

  • Franco, O.L.; Melo, F.R.; Mendes, P.A.; Paes, N.S.; Yokoyama, M.; Coutinho, M.V.; Bloch, C.; Grossi-de-Sa, M.F.
    Characterization of two Acanthoscelides obtectus alpha-amylases and their inactivation by wheat inhibitors (2005), J. Agric. Food Chem., 53, 1585-1590.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
alpha-AI1 alpha-amylase inhibitor 1 from cultivated Phaseolus vulgaris Acanthoscelides obtectus
alpha-AI2 alpha-amylase inhibitor 2 from wild Phaseolus vulgaris Acanthoscelides obtectus
wheat alpha-amylase inhibitor 0.19 inhibitor 0.19 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.19, overview Acanthoscelides obtectus
wheat alpha-amylase inhibitor 0.35 inhibitor 0.35 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.53, overview Acanthoscelides obtectus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Acanthoscelides obtectus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
isozyme AoA1, gel filtration Acanthoscelides obtectus
45000
-
isozyme AoA2, gel filtration Acanthoscelides obtectus

Organism

Organism UniProt Comment Textmining
Acanthoscelides obtectus
-
two isozymes AoA1 and AoA2
-

Purification (Commentary)

Purification (Comment) Organism
native isozymes AoA1 and AoA2 from larval gut 30.5fold and 65.4fold, respectively, by cation exchange and beta-cyclodextrin affinity chromatography, and gel filtration Acanthoscelides obtectus

Source Tissue

Source Tissue Comment Organism Textmining
gut
-
Acanthoscelides obtectus
-
larva
-
Acanthoscelides obtectus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
purified isozyme AoA1 and isozyme AoA2 Acanthoscelides obtectus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O soluble starch Acanthoscelides obtectus malto-oligosaccharides
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, isozyme AoA1, SDS-PAGE, 1 * 45000, isozyme AoA2, SDS-PAGE Acanthoscelides obtectus

Synonyms

Synonyms Comment Organism
AoA1
-
Acanthoscelides obtectus
AoA2
-
Acanthoscelides obtectus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
assay at Acanthoscelides obtectus