Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-AI1 | alpha-amylase inhibitor 1 from cultivated Phaseolus vulgaris | Acanthoscelides obtectus | |
alpha-AI2 | alpha-amylase inhibitor 2 from wild Phaseolus vulgaris | Acanthoscelides obtectus | |
wheat alpha-amylase inhibitor 0.19 | inhibitor 0.19 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.19, overview | Acanthoscelides obtectus | |
wheat alpha-amylase inhibitor 0.35 | inhibitor 0.35 isolated from wheat kernels, inhibits both isozymes AoA1 and AoA2 in vitro and in vivo, stoichiometry of inhibition of isozymes, biological activity of inhibitor 0.53, overview | Acanthoscelides obtectus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Acanthoscelides obtectus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
isozyme AoA1, gel filtration | Acanthoscelides obtectus |
45000 | - |
isozyme AoA2, gel filtration | Acanthoscelides obtectus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acanthoscelides obtectus | - |
two isozymes AoA1 and AoA2 | - |
Purification (Comment) | Organism |
---|---|
native isozymes AoA1 and AoA2 from larval gut 30.5fold and 65.4fold, respectively, by cation exchange and beta-cyclodextrin affinity chromatography, and gel filtration | Acanthoscelides obtectus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gut | - |
Acanthoscelides obtectus | - |
larva | - |
Acanthoscelides obtectus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified isozyme AoA1 and isozyme AoA2 | Acanthoscelides obtectus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | soluble starch | Acanthoscelides obtectus | malto-oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30000, isozyme AoA1, SDS-PAGE, 1 * 45000, isozyme AoA2, SDS-PAGE | Acanthoscelides obtectus |
Synonyms | Comment | Organism |
---|---|---|
AoA1 | - |
Acanthoscelides obtectus |
AoA2 | - |
Acanthoscelides obtectus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | Acanthoscelides obtectus |