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Literature summary for 3.2.1.1 extracted from

  • Kang, H.K.; Lee, J.H.; Kim, D.; Day, D.F.; Robyt, J.F.; Park, K.H.; Moon, T.W.
    Cloning and expression of Lipomyces starkeyi alpha-amylase in Escherichia coli and determination of some of its properties (2004), FEMS Microbiol. Lett., 233, 53-64.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene LSA, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli Lipomyces starkeyi

Inhibitors

Inhibitors Comment Organism Structure
acarbose
-
Lipomyces starkeyi
Cu2+
-
Lipomyces starkeyi

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates Lipomyces starkeyi
Mg2+ stimulates Lipomyces starkeyi
additional information no effect by 1 mM EGTA Lipomyces starkeyi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
68709
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi
73000
-
x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi

Organism

Organism UniProt Comment Textmining
Lipomyces starkeyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltodextrin G5 + H2O
-
Lipomyces starkeyi maltodextrin G2 + maltodextrin G3
-
?
maltodextrin G6 + H2O
-
Lipomyces starkeyi maltodextrin G2 + maltodextrin G4
-
?
maltodextrin G6 + H2O
-
Lipomyces starkeyi 2 maltodextrin G3
-
?
maltodextrin G7 + H2O
-
Lipomyces starkeyi maltodextrin G3 + maltodextrin G4
-
?
additional information no activity with maltodextrins of G2-G4 Lipomyces starkeyi ?
-
?
starch + H2O soluble starch Lipomyces starkeyi malto-oligosaccharides
-
?

Subunits

Subunits Comment Organism
? x * 68709, amino acid sequence calculation, x * 73000, recombinant His-tagged enzyme, SDS-PAGE Lipomyces starkeyi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Lipomyces starkeyi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lipomyces starkeyi

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 16 h, purified recombinant His-tagged enzyme, 90% stable Lipomyces starkeyi

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0034
-
acarbose pH 6.0, 40°C, recombinant His-tagged enzyme Lipomyces starkeyi