Cloned (Comment) | Organism |
---|---|
expresion of mutant enzymes in Escherichai coli strain MV1184 | Thermoactinomyces vulgaris |
Crystallization (Comment) | Organism |
---|---|
purified recombinant TVAII mutant enzyme D325N complexed with alpha-panosylpanose, hanging drop vapour diffusion method, 0.0015 ml of 20 mg/ml protein in 5 m Tris-HCl, pH 7.5, is mixed with 0.0015 ml of reservoir solution containing 1% w/v PEG 6000, 5 mM CaCl2, and 40 mM MES-NaOH, pH 6.1, soaking of crystals in cryoprotection solution containing 20% v/v 2-methyl-2,4-pentanediol, 20% w/v PEG 6000, and 2.5 mM CaCl2, X-ray diffraction structure determination and analysis at 2.2 A resolution, modelling | Thermoactinomyces vulgaris |
Protein Variants | Comment | Organism |
---|---|---|
D325N | site-directed mutagenesis, inactive mutant | Thermoactinomyces vulgaris |
Y374A | site-directed mutagenesis, the mutant shows reduced activity, and altered substrate specificity and kinetics compared to the wild-type enzyme | Thermoactinomyces vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic study | Thermoactinomyces vulgaris | |
0.19 | - |
starch | mutant Y374A | Thermoactinomyces vulgaris | |
0.23 | - |
starch | wild-type enzyme | Thermoactinomyces vulgaris | |
0.57 | - |
pullulan | mutant Y374A | Thermoactinomyces vulgaris | |
1.4 | - |
pullulan | wild-type enzyme | Thermoactinomyces vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces vulgaris | - |
- |
- |
Thermoactinomyces vulgaris R-47 | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(alpha-D-glucopyranosyl-(1-4))n-alpha-D-glucopyranose + H2O = (alpha-D-glucopyranosyl-(1-4))n-m-alpha-D-glucopyranose + (alpha-D-glucopyranosyl-(1-4))m-alpha-D-glucopyranose | TVAII is a bifunctional enzyme showing alpha-amylase as well as cyclodextrin-hydrolyzing activity, the enzyme hydrolyzes alpha-1,4-glucosidic linkages and alpha-1,6-glucosidic linkages, active site structure and substrate binding structure, Trp356 is involved in substrate binding, and Tyr374 is involved in substrate orientation for catalysis | Thermoactinomyces vulgaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermoactinomyces vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cyclodextrin + H2O | cyclomaltodextrinase activity, hydrolysis of alpha-1,4-glucosidic linkages | Thermoactinomyces vulgaris | panose + maltoheptaose | - |
? | |
isopanose + H2O | cyclomaltodextrinase activity, hydrolysis of alpha-1,6-glucosidic linkages | Thermoactinomyces vulgaris | ? | - |
? | |
additional information | substrate specificity of the bifunctional enzyme, the enzyme hydrolyzes alpha-1,4-glucosidic linkages and alpha-1,6-glucosidic linkages, and performs transglycosylation reactions, overview | Thermoactinomyces vulgaris | ? | - |
? | |
pullulan + H2O | cyclomaltodextrinase activity, hydrolysis of alpha-1,4-glucosidic linkages | Thermoactinomyces vulgaris | panose + ? | - |
? | |
starch + H2O | alpha-amylase activity, preferred substrate, hydrolysis of alpha-1,4-glucosidic linkages | Thermoactinomyces vulgaris | maltooligosaccharides | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-amylase II | - |
Thermoactinomyces vulgaris |
More | cf. EC 3.2.1.54, cyclomaltodextrinase | Thermoactinomyces vulgaris |
TVA II | - |
Thermoactinomyces vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.8 | - |
pullulan | mutant Y374A | Thermoactinomyces vulgaris | |
9.4 | - |
starch | mutant Y374A | Thermoactinomyces vulgaris | |
120 | - |
starch | wild-type enzyme | Thermoactinomyces vulgaris | |
140 | - |
pullulan | wild-type enzyme | Thermoactinomyces vulgaris |