Crystallization (Comment) | Organism |
---|---|
purified dimeric enzyme, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 50 mM Tris-HCl, pH 7.5, is mixed with 0.001 ml of precipitation solution, three different successful variations, overview, 1 week, X-ray diffraction structure determination and analysis at 3.0 A resolution | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 62000, glycosylated HSA, SDS-PAGE, x * 56000, deglycosylated HSA, SDS-PAGE | Homo sapiens |
62000 | - |
x * 62000, glycosylated HSA, SDS-PAGE, x * 56000, deglycosylated HSA, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
accidental co-purification of the native alpha-amylase with carbonic anhydrase VI from saliva by 4-aminobenzenesulfonamide affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
saliva | the enzyme is a major secretory protein component of the saliva | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malto-oligosaccharides + H2O | hydrolysis of alpha-1,4-glucosidic linkages | Homo sapiens | maltose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 62000, glycosylated HSA, SDS-PAGE, x * 56000, deglycosylated HSA, SDS-PAGE | Homo sapiens |
More | HSA consists of three domains, dimer structure, packing, and interface, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
HSA | - |
Homo sapiens |