Literature summary for 3.2.1.1 extracted from

Momma, M.; Fujimoto, Z.
Expression, crystallization and preliminary X-ray crystallographic studies of Klebsiella pneumoniae maltohexaose-producing alpha-amylase (2004), Acta Crystallogr. Sect. D, 60, 2352-2354.

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of the soluble enzyme in Escherichia coli	Klebsiella pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, microbatch technique by mixing of 0.0025 ml protein solution containing 10 mg/ml protein with 0.025 ml of precipitation solution, containing 80 mM sodium/potassium phosphate, pH 6.2, 8% PEG 3000, 4& PEG 3350, and 40 mM sodium thiocyanate, covering the mixture with 0.005 ml oil, 20°C, two weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution	Klebsiella pneumoniae

Crystallization (Comment)

Organism

purified recombinant enzyme, microbatch technique by mixing of 0.0025 ml protein solution containing 10 mg/ml protein with 0.025 ml of precipitation solution, containing 80 mM sodium/potassium phosphate, pH 6.2, 8% PEG 3000, 4& PEG 3350, and 40 mM sodium thiocyanate, covering the mixture with 0.005 ml oil, 20°C, two weeks, X-ray diffraction structure determination and analysis at 2.5 A resolution

Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
67000	-	x * 67000, recombinant enzyme, SDS-PAGE	Klebsiella pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Klebsiella pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli to homogeneity in 3 chromatographical steps	Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme is a maltohexaose-producing alpha-amylase	Klebsiella pneumoniae	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 67000, recombinant enzyme, SDS-PAGE	Klebsiella pneumoniae

Synonyms

Synonyms	Comment	Organism
G6-amylase	-	Klebsiella pneumoniae
maltohexaose-producing alpha-amylase	-	Klebsiella pneumoniae

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Release 2023.1 (January 2023)