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Literature summary for 3.2.1.1 extracted from
- Efficient expression, purification and characterization of mouse salivary alpha-amylase secreted from methylotrophic yeast, Pichia pastoris (2001), Yeast, 18, 643-655.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Mus musculus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 56269 | - |
x * 56269, MARDI-TOF mass spectrometry, non-glycosylated alpha-amylase | Mus musculus |
| 56286 | - |
x * 56286, deduced from nucleotide sequence | Mus musculus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| starch + H2O | Mus musculus | - |
fragments of starch | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | salivary alpha-amylase exists glycosylated and non-glycosylated form | Mus musculus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultrafiltration, DEAE-5PW, Phenyl-5PW | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| saliva | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 104.9 | - |
glycosylated alpha-amylase | Mus musculus |
| 229 | - |
non-glycosylated alpha-amylase | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenylpenta[alpha-D-glucopyranosyl(1-4)]-alpha-D-galactopyranoside | - |
Mus musculus | ? | - |
? | |
| starch + H2O | - |
Mus musculus | fragments of starch | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56269, MARDI-TOF mass spectrometry, non-glycosylated alpha-amylase | Mus musculus |
| ? | x * 56286, deduced from nucleotide sequence | Mus musculus |
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