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Literature summary for 3.2.1.1 extracted from

  • Ranwala, A.P.; Miller, W.B.
    Purification and characterization of an endoamylase from tulip (Tulipa gesneriana) bulbs (2000), Physiol. Plant., 109, 388-395.
No PubMed abstract available
Search for more literature for 3.2.1.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol 10 mM, slight activation Tulipa gesneriana

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-[2-nitrobenzoic acid] 10 mM, 35% inhibition Tulipa gesneriana
AgNO3 2 mM, complete inhibition Tulipa gesneriana
CuSO4 2 mM, 33% inhibition Tulipa gesneriana
EDTA 2 mM, 25% inhibition Tulipa gesneriana
HgCl2 2 mM, complete inhibition Tulipa gesneriana
N-ethylmaleimide 10 mM, 22% inhibition Tulipa gesneriana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 starch 1.28 mg/ml, 30°C, pH 6.0 Tulipa gesneriana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Tulipa gesneriana
51000
-
 1 * 51000, SDS-PAGE Tulipa gesneriana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Tulipa gesneriana
-
 fragments of starch
-
 ?

Organism

Organism UniProt Comment Textmining
Tulipa gesneriana
-
 L. cv. Apeldoorn
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, DEAE-Sephacel, Phenyl-agarose, Sephacryl S-200, chromatofocusing Tulipa gesneriana

Source Tissue

Source Tissue Comment Organism Textmining
bulb
-
 Tulipa gesneriana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
464
-
-
 Tulipa gesneriana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O
-
 Tulipa gesneriana fragments of amylopectin
-
 ?
amylose + H2O
-
 Tulipa gesneriana fragments of amylose
-
 ?
beta-limit dextrin + H2O
-
 Tulipa gesneriana fragments of beta-limit dextrin
-
 ?
glycogen + H2O 29% of activity with starch Tulipa gesneriana fragments of glycogen
-
 ?
starch + H2O
-
 Tulipa gesneriana fragments of starch
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 51000, SDS-PAGE Tulipa gesneriana

Synonyms

Synonyms Comment Organism
amylase I
-
 Tulipa gesneriana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Tulipa gesneriana

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 70 approx. 20% of maximal activity at 20°C, approx. 40% of maximal activity at 65°C Tulipa gesneriana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20
-
 approx. 20% loss of activity after 30 min, approx. 10% loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana
30
-
 approx. 30% loss of activity after 30 min, approx. 15% loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana
40
-
 approx. 50% loss of activity after 30 min, approx. 25% loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana
50
-
 almost complete loss of activity after 30 min, approx. 30% loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana
60
-
 approx. 65% loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana
70
-
 almost complete loss of activity in the presence of 5 mM CaCl2 Tulipa gesneriana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Tulipa gesneriana

pH Range

pH Minimum pH Maximum Comment Organism
3.5 9 approx. 30% of maximal activity at pH 3.5, approx. 20% of maximal activity at pH 9.0 Tulipa gesneriana