Literature summary for 3.2.1.1 extracted from

Chang, C.T.; Lo, H.F.; Chi, M.C.; Yao, C.Y.; Hsu, W.H.; Lin, L.L.
Identification of essential histidine residues in a recombinant alpha-amylase of thermophilic and alkalophilic Bacillus sp. strain TS-23 (2003), Extremophiles, 7, 505-509.

Search for more literature for 3.2.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
H137L	no activity	Bacillus sp. (in: Bacteria)
H191L	similar activity as wild-type	Bacillus sp. (in: Bacteria)
H239L	similar activity as wild-type	Bacillus sp. (in: Bacteria)
H269L	no activity	Bacillus sp. (in: Bacteria)
H305L	similar activity as wild-type	Bacillus sp. (in: Bacteria)
H323L	approx. 50% of wild-type activity	Bacillus sp. (in: Bacteria)
H361L	no activity	Bacillus sp. (in: Bacteria)
H436L	approx. 40% of wild-type activity, reduced thermostability	Bacillus sp. (in: Bacteria)
H475L	similar activity as wild-type	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	starch	2.9 mg/ml	Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
54000	-	x * 54000, SDS-PAGE, recombinant alpha-amylase	Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
starch + H2O	Bacillus sp. (in: Bacteria)	-	fragments of starch	-	?
starch + H2O	Bacillus sp. (in: Bacteria) TS-23	-	fragments of starch	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	-	-	-
Bacillus sp. (in: Bacteria) TS-23	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant alpha-amylase	Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
187	-	-	Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
starch + H2O	-	Bacillus sp. (in: Bacteria)	fragments of starch	-	?
starch + H2O	-	Bacillus sp. (in: Bacteria) TS-23	fragments of starch	-	?

Subunits

Subunits	Comment	Organism
?	x * 54000, SDS-PAGE, recombinant alpha-amylase	Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
70	-	approx. 40% loss of activity after 30 min	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
168.5	-	starch	-	Bacillus sp. (in: Bacteria)

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Release 2023.1 (January 2023)