Cloned (Comment) | Organism |
---|---|
expression in Sf9 insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
crystals of wild-type and F256W mutant alpha-amylase, crystals diffract to 2.1 a resolution | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, F256W mutant | Homo sapiens | |
0.27 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, wild-type | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, non-glycosylated alpha-amylase | Homo sapiens |
62000 | - |
x * 62000, glycosylated alpha-amylase | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Homo sapiens | - |
fragments of starch | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | human salivary alpha-amylase occurs in both glycosylated and non-glycosylated forms | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant alpha-amylase | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
saliva | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
F256W mutant, 900 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min | Homo sapiens |
additional information | - |
wild-type, 66400 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O | - |
Homo sapiens | ? | - |
? | |
starch + H2O | - |
Homo sapiens | fragments of starch | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, non-glycosylated alpha-amylase | Homo sapiens |
? | x * 62000, glycosylated alpha-amylase | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.5 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, F256W mutant | Homo sapiens | |
175 | - |
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside | 37°C, pH 8.0, wild-type | Homo sapiens |