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Literature summary for 3.2.1.1 extracted from

  • Ramasubbu, N.; Sundar, K.; Ragunath, C.; Rafi, M.M.
    Structural studies of a Phe256Trp mutant of human salivary alpha-amylase: implications for the role of a conserved water molecule in enzyme activity (2004), Arch. Biochem. Biophys., 421, 115-124.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Sf9 insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of wild-type and F256W mutant alpha-amylase, crystals diffract to 2.1 a resolution Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, F256W mutant Homo sapiens
0.27
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, wild-type Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, non-glycosylated alpha-amylase Homo sapiens
62000
-
x * 62000, glycosylated alpha-amylase Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Homo sapiens
-
fragments of starch
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein human salivary alpha-amylase occurs in both glycosylated and non-glycosylated forms Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant alpha-amylase Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
saliva
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
F256W mutant, 900 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min Homo sapiens
additional information
-
wild-type, 66400 units, 1 unit is definded as the amount of alpha-amylase able to decrease 1% of absorbance at 620 nm in 1 min Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside + H2O
-
Homo sapiens ?
-
?
starch + H2O
-
Homo sapiens fragments of starch
-
?

Subunits

Subunits Comment Organism
? x * 55000, non-glycosylated alpha-amylase Homo sapiens
? x * 62000, glycosylated alpha-amylase Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.5
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, F256W mutant Homo sapiens
175
-
p-nitrophenylhexa[alpha-D-glucopyranosyl(1-4)]-alpha-D-glucopyranoside 37°C, pH 8.0, wild-type Homo sapiens