General Stability | Organism |
---|---|
at 65°C the enzyme is nearly 8times more stable in presence of Ca2+. Ca2+ increases the melting temperature from 66°C to 73°C | Thermomyces lanuginosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Thermomyces lanuginosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermomyces lanuginosus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
calculation of the molecular weight from relationship of retardation coefficient versus molecular weight of marker proteins | Thermomyces lanuginosus |
72000 | - |
gel filtration | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | - |
- |
- |
Thermomyces lanuginosus IISc91 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 5% carbohydrate | Thermomyces lanuginosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Thermomyces lanuginosus | additional information | production of high levels of maltose | ? | |
starch + H2O | - |
Thermomyces lanuginosus IISc91 | additional information | production of high levels of maltose | ? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
at 65°C the enzyme is nearly 8times more stable in presence of Ca2+ | Thermomyces lanuginosus |
50 | - |
stable for more than 7 h | Thermomyces lanuginosus |
65 | - |
half-life: 0.6 h | Thermomyces lanuginosus |
66 | - |
melting temperature in absence of Ca2+ | Thermomyces lanuginosus |
73 | - |
melting temperature in presence of Ca2+ | Thermomyces lanuginosus |
94 | - |
irreversible inactivation by conversion into an inactive 72 kDa trimer | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.6 | - |
- |
Thermomyces lanuginosus |