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Literature summary for 3.2.1.1 extracted from

  • Mishra, R.S.; Maheshwari, R.
    Amylases of the thermophilic fungus Thermomyces lanuginosus: their purification, properties, action on starch and response to heat (1996), J. Biosci., 21, 653-672.
No PubMed abstract available

General Stability

General Stability Organism
at 65°C the enzyme is nearly 8times more stable in presence of Ca2+. Ca2+ increases the melting temperature from 66°C to 73°C Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Thermomyces lanuginosus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomyces lanuginosus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
calculation of the molecular weight from relationship of retardation coefficient versus molecular weight of marker proteins Thermomyces lanuginosus
72000
-
gel filtration Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-
Thermomyces lanuginosus IISc91
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 5% carbohydrate Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
starch + H2O
-
Thermomyces lanuginosus additional information production of high levels of maltose ?
starch + H2O
-
Thermomyces lanuginosus IISc91 additional information production of high levels of maltose ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
at 65°C the enzyme is nearly 8times more stable in presence of Ca2+ Thermomyces lanuginosus
50
-
stable for more than 7 h Thermomyces lanuginosus
65
-
half-life: 0.6 h Thermomyces lanuginosus
66
-
melting temperature in absence of Ca2+ Thermomyces lanuginosus
73
-
melting temperature in presence of Ca2+ Thermomyces lanuginosus
94
-
irreversible inactivation by conversion into an inactive 72 kDa trimer Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
Thermomyces lanuginosus