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Literature summary for 3.2.1.1 extracted from

  • Oosthuizen, V.; Naude, R.J.; Oelofsen, W.; Muramoto, K.; Kamiya, H.
    Ostrich, pancreatic alpha-amylase: kinetic properties, amino terminal sequence and subsite structure (1994), Int. J. Biochem., 26, 1313-1321.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2-deoxyglucose
-
Struthio camelus
alpha-D-methylglucopyranose
-
Struthio camelus
beta-cyclodextrin
-
Struthio camelus
D-gluconic acid lactone
-
Struthio camelus
D-glucosamine
-
Struthio camelus
wheat amylase inhibitor
-
Struthio camelus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.43
-
maltohexaose
-
Struthio camelus
0.51
-
maltoheptaose
-
Struthio camelus
0.65 1.33 maltotetraose value depends on assay method Struthio camelus
0.95
-
maltopentaose
-
Struthio camelus
3.57
-
maltotriose
-
Struthio camelus
26.16
-
maltose
-
Struthio camelus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
x * 52000, SDS-PAGE Struthio camelus

Organism

Organism UniProt Comment Textmining
Struthio camelus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Struthio camelus

Source Tissue

Source Tissue Comment Organism Textmining
pancreas
-
Struthio camelus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltoheptaose + H2O
-
Struthio camelus additional information maltotetraose + maltotriose ?
maltohexaose + H2O
-
Struthio camelus additional information maltose + maltotetraose or maltotriose ?
maltopentaose + H2O
-
Struthio camelus additional information maltose + maltotriose ?
maltose + H2O
-
Struthio camelus ?
-
?
maltotetraose + H2O
-
Struthio camelus additional information
-
?
maltotriose + H2O
-
Struthio camelus maltose + D-glucose
-
?

Subunits

Subunits Comment Organism
? x * 52000, SDS-PAGE Struthio camelus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.23
-
maltose
-
Struthio camelus
0.81
-
maltotriose
-
Struthio camelus
117 144 maltotetraose value depends on assay method Struthio camelus
580
-
maltohexaose
-
Struthio camelus
679
-
maltoheptaose
-
Struthio camelus
754
-
maltopentaose
-
Struthio camelus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
hydrolysis of maltopentaose, maltohexaose and maltoheptaose Struthio camelus