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Literature summary for 3.2.1.1 extracted from
- Purification and properties of alpha-amylase from Aspergillus oryzae ATCC 76080 (1995), Biochem. Mol. Biol. Int., 36, 185-193.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-Dinitro-1-fluorobenzene | 6 mM | Aspergillus oryzae |  |
| Hg2+ | 0.3 mM | Aspergillus oryzae | |
| N-Acetylimidazole | 6 mM | Aspergillus oryzae | |
| N-bromosuccinimide | 6 mM | Aspergillus oryzae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus oryzae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
gel filtration | Aspergillus oryzae |
| 54000 | - |
1 * 54000, SDS-PAGE | Aspergillus oryzae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus oryzae | - |
ATCC 76080 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus oryzae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 410 | - |
- |
Aspergillus oryzae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltotetraose + H2O | - |
Aspergillus oryzae | additional information | maltose with some glucose and maltotriose | ? | |
| maltotriose + H2O | - |
Aspergillus oryzae | maltose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, SDS-PAGE | Aspergillus oryzae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus oryzae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 5, 30 min, stable below | Aspergillus oryzae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5 | - |
Aspergillus oryzae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | 30 min, stable | Aspergillus oryzae |
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Release 2023.1 (January 2023)
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