Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.1 extracted from

  • Anindyawati, T.; Melliawati, R.; Ito, K.; Iizuka, M.; Minamiura, N.
    Three Different Types of ?-Amylases from Aspergillus awamori KT-11: Their Purifications, Properties, and Specificities. (1998), Biosci. Biotechnol. Biochem., 62, 1351-1357.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 10 mM CaCl2, inhibition of enzyme form Amyl II and Amyl III, no inhibition of enzyme form Amyl I Aspergillus awamori
Cu2+ 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III Aspergillus awamori
Hg2+ 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III Aspergillus awamori
Zn2+ 10 mM, strong inhibition of enzyme form Amyl I, Amyl II and Amyl III Aspergillus awamori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Aspergillus awamori

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ activates Aspergillus awamori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 enzyme form Amyl I, gel filtration Aspergillus awamori
59000
-
 enzyme form Amyl II, gel filtration Aspergillus awamori
120000
-
 enzyme form Amyl III, gel filtration Aspergillus awamori

Organism

Organism UniProt Comment Textmining
Aspergillus awamori
-
-
-
Aspergillus awamori KT-11
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme form Amyl I contains 10.4% carbohydrate, enzyme form Amyl II contains 6.3% carbohydrate and enzyme form Amyl III 11.3% carbohydrate Aspergillus awamori

Purification (Commentary)

Purification (Comment) Organism
three enzyme forms: Amyl I, Amyl II and Amyl III Aspergillus awamori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cyclodextrin + H2O
-
 Aspergillus awamori ?
-
 ?
maltoheptaose + H2O
-
 Aspergillus awamori additional information
-
 ?
maltohexaose + H2O
-
 Aspergillus awamori additional information
-
 ?
maltohexaose + H2O
-
 Aspergillus awamori KT-11 additional information
-
 ?
maltopentaose + H2O
-
 Aspergillus awamori additional information
-
 ?
maltopentaose + H2O
-
 Aspergillus awamori KT-11 additional information
-
 ?
maltotetraose + H2O
-
 Aspergillus awamori additional information
-
 ?
maltotetraose + H2O
-
 Aspergillus awamori KT-11 additional information
-
 ?
starch + H2O soluble starch Aspergillus awamori additional information enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose ?
starch + H2O waxy corn starch Aspergillus awamori additional information enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose ?
starch + H2O soluble starch Aspergillus awamori KT-11 additional information enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose ?
starch + H2O waxy corn starch Aspergillus awamori KT-11 additional information enzyme form Amyl I hydrolyzes waxy maize starch to produce mainly maltose, maltotriose, and some maltooligosaccharides with higher degree of polymerization, the enzyme forms Amyl II and Amyl III produce maltose and some maltooligosaccharides of higher molecular weight than maltotetraose ?

Subunits

Subunits Comment Organism
monomer 1 * 59000, enzyme form Amyl II, SDS-PAGE Aspergillus awamori
monomer 1 * 120000, enzyme form Amyl III, SDS-PAGE Aspergillus awamori
monomer 1 * 46000, enzyme form Amyl I, SDS-PAGE Aspergillus awamori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 enzyme form Amyl I Aspergillus awamori
60
-
 enzyme form Amyl II and Amyl III Aspergillus awamori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 enzyme form Amyl I Aspergillus awamori
5.5
-
 enzyme form Amyl II and Amyl III Aspergillus awamori