Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, 23% loss of activity | Streptomyces megasporus | |
Ag+ | 1 mM AgCl2, 77% loss of activity | Streptomyces megasporus | |
Ba2+ | 1 mM BaCl2, 40% loss of activity | Streptomyces megasporus | |
Co2+ | 1 mM CoCl2, 65% loss of activity | Streptomyces megasporus | |
Cu2+ | 1 mM CuCl2, 28% loss of activity | Streptomyces megasporus | |
EDTA | 1 mM, 85% loss of activity | Streptomyces megasporus | |
Fe2+ | 1 mM FeCl2, 38% loss of activity | Streptomyces megasporus | |
Hg2+ | 1 mM HgCl2, 88% inhibition | Streptomyces megasporus | |
Mg2+ | 1 mM MgCl2, 30% loss of activity | Streptomyces megasporus | |
Mn2+ | 1 mM, 55% loss of activity | Streptomyces megasporus | |
Pb2+ | 1 mM, 50% loss of activity | Streptomyces megasporus | |
PCMB | 1 mM, 92% loss of activity | Streptomyces megasporus | |
Zn2+ | 1 mM ZnCl2, 45% loss of activity | Streptomyces megasporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 4.4 mg/ml for starch | Streptomyces megasporus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Streptomyces megasporus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
x * 97000, SDS-PAGE | Streptomyces megasporus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces megasporus | - |
- |
- |
Streptomyces megasporus SD12 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Streptomyces megasporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | - |
Streptomyces megasporus | additional information | major products are maltotriose and maltose | ? | |
starch + H2O | - |
Streptomyces megasporus | additional information | maltose and traces of maltotriose | ? | |
starch + H2O | - |
Streptomyces megasporus SD12 | additional information | major products are maltotriose and maltose | ? | |
starch + H2O | - |
Streptomyces megasporus SD12 | additional information | maltose and traces of maltotriose | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 97000, SDS-PAGE | Streptomyces megasporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces megasporus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 75 | 45°C: about 50% of maximal activity, 75°C: about 60% of maximal activity | Streptomyces megasporus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
half-life: 2 h | Streptomyces megasporus |
80 | - |
half-life: 90 min | Streptomyces megasporus |
85 | - |
half-life: 60 min | Streptomyces megasporus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3000 | - |
starch | calculated as low molecular weight products | Streptomyces megasporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Streptomyces megasporus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7.5 | pH 5.0: about 60% of maximal activity, pH 7.5: about 50% of maximal activity | Streptomyces megasporus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | stable | Streptomyces megasporus |