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Literature summary for 3.2.1.1 extracted from

  • von Tigerstrom, R.G.; Stelmaschuk, S.
    Purification and partial characterization of an amylase from Lysobacter brunescens (1987), J. Gen. Microbiol., 133, 3437-3443.
No PubMed abstract available
Search for more literature for 3.2.1.1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 1 * 47000, SDS-PAGE Lysobacter brunescens
49000
-
 glycerol density gradient centrifugation Lysobacter brunescens

Organism

Organism UniProt Comment Textmining
Lysobacter brunescens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysobacter brunescens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
371
-
-
 Lysobacter brunescens

Storage Stability

Storage Stability Organism
0°C or -20°C, 25 mM Tris-HCl, 100 mM KCl, pH 7.5 or 50 mM Tris-HCl, pH 7.5, stable for at least 9 days Lysobacter brunescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 95% of the activity obtained with starch Lysobacter brunescens additional information
-
 ?
amylose + H2O 65% of the activity obtained with starch Lysobacter brunescens additional information
-
 ?
glycogen + H2O 65% of the activity obtained with starch Lysobacter brunescens additional information
-
 ?
starch + H2O
-
 Lysobacter brunescens additional information
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 47000, SDS-PAGE Lysobacter brunescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 20 min, 67% loss of activity, 20% loss of activity in presence of 2 mM CaCl2 or 0.5 mg bovine serum albumin Lysobacter brunescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 7.5
-
 Lysobacter brunescens