Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, gel filtration | Geobacillus stearothermophilus |
58000 | - |
x * 58000, SDS-PAGE | Geobacillus stearothermophilus |
61500 | - |
the difference between the value determined by gel filtration and by nondenaturing gel electrophoresisis is due to hydrophobic interactions between the chromatography matrix and the enzyme molecule, nondenaturing gel electrophoresis | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltodextrin + H2O | - |
Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | hydrolysis of maltodextrin | Geobacillus stearothermophilus |
80 | - |
hydrolysis of starch | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life in presence of 0.05 mM Ca2+ is 4.3 min. Half-life in presence of 5 mM Ca2+ is 44 min. Half-life in presence of 10% dextrose and 5 mM Ca2+ at pH 5.35 is 51 min. Half-life in presence of 10% maltose and 5 mM Ca2+ at pH 5.35 is 55 min. Half-life in presence of 10% maltotriose and 5 mM Ca2+ at pH 5.35 is 166 min. Half-life in presence of 10% Maltrin M-105 and 5 mM Ca2+ at pH 5.35 is 342 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 7 | at 40°C | Geobacillus stearothermophilus |
5.2 | 6.5 | at 90°C | Geobacillus stearothermophilus |