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Literature summary for 3.2.1.1 extracted from

  • Ohno, N.; Ijuin, T.; Song, S.; Uchiyama, S.; Shinoyama, H.; Ando, A.; Fujii, T.
    Purification and Properties of Amylases Extracellularly Produced by an Imperfect Fungus, Fusidium sp. BX-1 in a Glycerol Medium. (1992), Biosci. Biotechnol. Biochem., 56, 465-471.
    View publication on PubMed
Search for more literature for 3.2.1.1

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 1 mM, 28% inhibition Fusidium sp.
Hg2+ 1 mM, 66% inhibition Fusidium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Fusidium sp.
1.9
-
 maltohexaose
-
 Fusidium sp.
2.7
-
 maltopentaose
-
 Fusidium sp.
8.3
-
 maltotetraose
-
 Fusidium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Fusidium sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000 53000 gel filtration Fusidium sp.

Organism

Organism UniProt Comment Textmining
Fusidium sp.
-
-
-
Fusidium sp. BX-1
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 3.4% carbohydrate Fusidium sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Fusidium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Fusidium sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltohexaose + H2O
-
 Fusidium sp. additional information
-
 ?
maltohexaose + H2O
-
 Fusidium sp. BX-1 additional information
-
 ?
maltopentaose + H2O
-
 Fusidium sp. additional information
-
 ?
maltopentaose + H2O
-
 Fusidium sp. BX-1 additional information
-
 ?
maltotetraose + H2O
-
 Fusidium sp. additional information
-
 ?
maltotetraose + H2O
-
 Fusidium sp. BX-1 additional information
-
 ?
starch + H2O soluble starch Fusidium sp. additional information main products are maltose and maltotriose, minor products are maltotetraose and maltopentaose in the early stage of reaction. Small amounts of glucose are detected after 24 h ?
starch + H2O soluble starch Fusidium sp. BX-1 additional information main products are maltose and maltotriose, minor products are maltotetraose and maltopentaose in the early stage of reaction. Small amounts of glucose are detected after 24 h ?

Subunits

Subunits Comment Organism
monomer
-
 Fusidium sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50 55
-
 Fusidium sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 30°C: about 50% of maximal activity, 70°C: about 25% of maximal activity Fusidium sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 1 h, stable below Fusidium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Fusidium sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 pH 3.0: about 80% of maximal activity, pH 5.5: about 50% of maximal activity Fusidium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 30°C, 30 min, stable Fusidium sp.