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Literature summary for 3.2.1.1 extracted from
- Purification and characterization of extracellular alpha-amylase and glucoamylase from the yeast Candida antarctica CBS 6678 (1987), Eur. J. Biochem., 164, 643-654.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acarbose | - |
Moesziomyces antarcticus |  |
| alpha-cyclodextrin | - |
Moesziomyces antarcticus | |
| Bay e4609 | - |
Moesziomyces antarcticus | |
| beta-cyclodextrin | - |
Moesziomyces antarcticus | |
| maltitol | - |
Moesziomyces antarcticus | |
| maltose | - |
Moesziomyces antarcticus | |
| trestatin A | - |
Moesziomyces antarcticus | |
| trestatin B | - |
Moesziomyces antarcticus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.77 | - |
amylose | short chain | Moesziomyces antarcticus | |
| 2.7 | - |
maltoheptaose | - |
Moesziomyces antarcticus | |
| 3.1 | - |
maltopentaose | - |
Moesziomyces antarcticus | |
| 3.3 | - |
starch | soluble starch, Merck, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 3.5 | - |
amylopectin | calculated as low molecular weight products | Moesziomyces antarcticus | |
| 4.3 | - |
starch | soluble starch, Zulkowsky type starch, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 4.4 | - |
gamma-cyclodextrin | - |
Moesziomyces antarcticus | |
| 6.5 | - |
maltotriose | - |
Moesziomyces antarcticus | |
| 13.6 | - |
beta-cyclodextrin | - |
Moesziomyces antarcticus | |
| 36.4 | - |
glycogen | from rat liver, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 51.1 | - |
glycogen | from oyster, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 52 | - |
alpha-cyclodextrin | - |
Moesziomyces antarcticus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Moesziomyces antarcticus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | 48000 | gel filtration | Moesziomyces antarcticus |
| 51000 | - |
1 * 51000, SDS-PAGE | Moesziomyces antarcticus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Moesziomyces antarcticus | - |
- |
- |
| Moesziomyces antarcticus CBS 6678 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Moesziomyces antarcticus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Moesziomyces antarcticus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cyclodextrin + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
| amylopectin + H2O | - |
Moesziomyces antarcticus | additional information | - |
? | |
| amylose + H2O | short chain amylose | Moesziomyces antarcticus | additional information | - |
? | |
| beta-cyclodextrin + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
| cyclodextrin + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
| gamma-cyclodextrin + H2O | - |
Moesziomyces antarcticus | ? | - |
? | |
| glycogen + H2O | - |
Moesziomyces antarcticus | additional information | - |
? | |
| maltoheptaose + H2O | - |
Moesziomyces antarcticus | additional information | - |
? | |
| maltopentaose + H2O | - |
Moesziomyces antarcticus | additional information | - |
? | |
| maltotriose + H2O | - |
Moesziomyces antarcticus | maltose + D-glucose | - |
? | |
| starch + H2O | soluble starch | Moesziomyces antarcticus | additional information | - |
? | |
| starch + H2O | raw starches | Moesziomyces antarcticus | additional information | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Moesziomyces antarcticus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 62 | - |
- |
Moesziomyces antarcticus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 75 | 37°C: about 65% of maximal activity, 75°C: about 65% of maximal activity | Moesziomyces antarcticus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.6 | - |
maltotriose | - |
Moesziomyces antarcticus | |
| 10.7 | - |
alpha-cyclodextrin | calculated as low molecular weight products | Moesziomyces antarcticus | |
| 29.4 | - |
beta-cyclodextrin | calculated as low molecular weight products | Moesziomyces antarcticus | |
| 94.7 | - |
maltopentaose | - |
Moesziomyces antarcticus | |
| 105 | - |
maltoheptaose | - |
Moesziomyces antarcticus | |
| 130 | - |
glycogen | from rat liver, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 166 | - |
glycogen | from oyster, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 171 | - |
amylopectin | calculated as low molecular weight products | Moesziomyces antarcticus | |
| 186 | - |
gamma-cyclodextrin | calculated as low molecular weight products | Moesziomyces antarcticus | |
| 206 | - |
starch | soluble starch of Zulkowsky type, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 230 | - |
amylose | short chain amylose, calculated as low molecular weight products | Moesziomyces antarcticus | |
| 237 | - |
starch | soluble starch from Merck, calculated as low molecular weight products | Moesziomyces antarcticus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.2 | - |
- |
Moesziomyces antarcticus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 6 | pH 2.5: about 25% of maximal activity, pH 6.0: about 60% of maximal activity | Moesziomyces antarcticus |
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