Crystallization (Comment) | Organism |
---|---|
mutant enzymes | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
G50A | kcat/Km is 12820fold lower than wild-type value | Bacillus cereus |
G50P | inactive mutant protein | Bacillus cereus |
G50V | inactive mutant protein | Bacillus cereus |
H56Q | kcat/Km is 238fold lower than wild-type value | Bacillus cereus |
K53A | inactive mutant protein | Bacillus cereus |
K53R | inactive mutant protein | Bacillus cereus |
M49L | kcat/Km is 17241fold lower than wild-type value | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, wild-type enzyme | Bacillus cereus | |
0.175 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme H56Q | Bacillus cereus | |
5.1 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme m49L | Bacillus cereus | |
11 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme G50A | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Renatured (Comment) | Organism |
---|---|
expression of mutant enzymes in escherichia coli | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphonoacetaldehyde + H2O | catalysis within the core domain of phosphonatase requires the participation of loop 5 of the corresponding cap domain. Gly is an indispensable component | Bacillus cereus | acetaldehyde + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phosphonatase | - |
Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00247 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme m49L | Bacillus cereus | |
0.006 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme H56Q | Bacillus cereus | |
0.0071 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, mutant enzyme G50A | Bacillus cereus | |
0.25 | - |
phosphonoacetaldehyde | 25°C, pH 7.5, wild-type enzyme | Bacillus cereus |