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Literature summary for 3.1.6.8 extracted from
- Interaction of arylsulfatase-A (ASA) with its natural sulfoglycolipid substrates: a computational and site-directed mutagenesis study (2009), Glycoconj. J., 26, 1029-1045.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in CHO-K1 cells | Sus scrofa |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C69A | site-directed mutagenesis, the mnutant shows abolished enzyme activity and only residual binding to thr sulfoglycolipid substrate | Sus scrofa |
| C69A/K123A/K302A | site-directed mutagenesis, the mutant shows abolished substrate binding and activity | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-methylumbelliferyl phosphate | - |
Homo sapiens |  |
| 4-methylumbelliferyl phosphate | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| acrosome | - |
Homo sapiens | - |
- |
| acrosome | - |
Sus scrofa | - |
- |
| lysosome | - |
Homo sapiens | 5764 | - |
| lysosome | - |
Sus scrofa | 5764 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sulfogalactosylceramide + H2O | Homo sapiens | - |
? | - |
? | |
| sulfogalactosylceramide + H2O | Sus scrofa | - |
? | - |
? | |
| sulfogalactosylglycerolipid + H2O | Homo sapiens | - |
? | - |
? | |
| sulfogalactosylglycerolipid + H2O | Sus scrofa | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | P15289 | - |
- |
| Sus scrofa | Q8WNR3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
| sperm | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
| testis | - |
Sus scrofa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrocatechol sulfate + H2O | artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Homo sapiens | 4-nitrocatechol + sulfate | - |
? | |
| 4-nitrocatechol sulfate + H2O | artificial substrate, Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Sus scrofa | 4-nitrocatechol + sulfate | - |
? | |
| additional information | substrate binding and docking study, using crystal structures, PDB IDs 1N2K and 1E2S, overview | Homo sapiens | ? | - |
? | |
| additional information | substrate binding and docking study, using human crystal structures, PDB IDs 1N2K and 1E2S, overview | Sus scrofa | ? | - |
? | |
| sulfogalactosylceramide + H2O | - |
Homo sapiens | ? | - |
? | |
| sulfogalactosylceramide + H2O | - |
Sus scrofa | ? | - |
? | |
| sulfogalactosylceramide + H2O | Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Homo sapiens | ? | - |
? | |
| sulfogalactosylceramide + H2O | Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Sus scrofa | ? | - |
? | |
| sulfogalactosylglycerolipid + H2O | - |
Homo sapiens | ? | - |
? | |
| sulfogalactosylglycerolipid + H2O | - |
Sus scrofa | ? | - |
? | |
| sulfogalactosylglycerolipid + H2O | Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Homo sapiens | ? | - |
? | |
| sulfogalactosylglycerolipid + H2O | Cys69 is the catalytic residue, and Lys302 and Lys123 act as residues anchoring the sulfate group to the active site, interaction with the enzyme active site, overview | Sus scrofa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylsulfatase-A | - |
Homo sapiens |
| arylsulfatase-A | - |
Sus scrofa |
| ASA | - |
Homo sapiens |
| ASA | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
| 37 | - |
assay at | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Homo sapiens |
| 5 | - |
- |
Sus scrofa |
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