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Literature summary for 3.1.6.8 extracted from

  • Sarosiek, B.; Ciereszko, A.; Kolman, R.; Glogowski, J.
    Characteristics of arylsulfatase in Russian sturgeon (Acipenser gueldenstaedti) semen (2004), Comp. Biochem. Physiol. B, 139, 571-579.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 50% decreased activity in milt plasma with 60 nM AgNO3 and 50% decreased activity in spermatozoon with 4 nM AgNO3 Acipenser gueldenstaedtii
H2SO4 50% decreased activity in milt plasma with 25 mM H2SO4 and 26% decreased activity in spermatozoon with 25 mM H2SO4 Acipenser gueldenstaedtii
H3PO4 56% decreased activity in milt plasma with 1 mM H3PO4 and 43% decreased activity in spermatozoon with 1 mM H2SO4 Acipenser gueldenstaedtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.175
-
p-nitrocatechol sulfate in milt plasma with 60 nM AgNO3 Acipenser gueldenstaedtii
0.3
-
p-nitrocatechol sulfate in milt plasma without sodium chloride Acipenser gueldenstaedtii
0.45
-
p-nitrocatechol sulfate in milt plasma with 160 mM sodium chloride Acipenser gueldenstaedtii
0.81
-
p-nitrocatechol sulfate in spermatozoon with 160 mM sodium chloride Acipenser gueldenstaedtii
1
-
p-nitrocatechol sulfate in spermatozoon without sodium chloride Acipenser gueldenstaedtii

Metals/Ions

Metals/Ions Comment Organism Structure
Cl- activation of milt plasma arylsulfatase A is increased up to 78% after treatment with 160 mM NaCl, activation of spermatozoon arylsulfatase A is increased up to 147% after treatment with 160 mM NaCl Acipenser gueldenstaedtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
172000
-
gel-filtration Acipenser gueldenstaedtii

Organism

Organism UniProt Comment Textmining
Acipenser gueldenstaedtii
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
spermatozoon
-
Acipenser gueldenstaedtii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.633
-
spermatozoon, 0.5 M acetate buffer, pH 4.9, 37°C Acipenser gueldenstaedtii
4.316
-
milt plasma, 0.5 M acetate buffer, pH 4.9, 37°C Acipenser gueldenstaedtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrocatechol sulfate + H2O
-
Acipenser gueldenstaedtii p-nitrocatechol + sulfate
-
?

Subunits

Subunits Comment Organism
dimer 2 x 77000, SDS-PAGE Acipenser gueldenstaedtii

Synonyms

Synonyms Comment Organism
arylsulfatase A
-
Acipenser gueldenstaedtii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
milt plasma and spermatozoon Acipenser gueldenstaedtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.9
-
in milt plasma Acipenser gueldenstaedtii
5
-
in spermatozoon Acipenser gueldenstaedtii