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Literature summary for 3.1.6.2 extracted from
- Dehydroepiandrosterone sulphate sulphohydrolase from human placenta microsomes - properties of the purified enzyme (2006), J. Steroid Biochem. Mol. Biol., 99, 67-75.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glutathione | oxidized form, 5 mM, 187% of initial activity | Homo sapiens |  |
| L-beta-phenylalanine | 5 mM, 169% of initial activity | Homo sapiens | |
| L-cysteine | 5 mM, 202% of initial activity | Homo sapiens | |
| lithocholic acid | 5 mM, 226% of initial activity | Homo sapiens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ammonium molybdate | 10 mM, 20% residual activity | Homo sapiens | |
| calcium chloride | 5 mM, 36% residual activity | Homo sapiens | |
| dithiothreitol | 5 mM, 45% residual activity | Homo sapiens | |
| glutathione | reduced form, 5 mM, 23.5% residual activity | Homo sapiens | |
| additional information | not inhibitory: phosphate, sulfate, sulfide | Homo sapiens | |
| vanadium oxide(V) | 50 mM, 51% residual activity | Homo sapiens | |
| Zinc acetate | 1.25 mM, 53% residual activity | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
dehydroepiandrosterone 3-sulfate | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Homo sapiens | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, SDS-PAGE | Homo sapiens |
| 74000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
enzyme does not copurify with oestrone sulfate sulfohydrolase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme does not copurify with oestrone sulfate sulfohydrolase | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.52 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dehydroepiandrosterone 3-sulfate + H2O | - |
Homo sapiens | dehydroepiandrosterone + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
5 min, 115% resiudal activity | Homo sapiens |
| 80 | - |
5 min, 100% residual activity | Homo sapiens |
| 100 | - |
5 min, 87% residual activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Homo sapiens |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Homo sapiens | isoelectric focusing | - |
8.7 |
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