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Literature summary for 3.1.6.2 extracted from
- Solubilization and partial purification of steroid sulfatase from rat liver: characterization of estrone sulfatase (1976), Arch. Biochem. Biophys., 174, 199-208.
General Stability
| General Stability | Organism |
|---|---|
| lyophilization, deactivation | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | slight | Rattus norvegicus |  |
| AgNO3 | slight | Rattus norvegicus | |
| CaCl2 | slight | Rattus norvegicus | |
| EDTA | 10% inhibition at 1 mM | Rattus norvegicus | |
| KBr | slight | Rattus norvegicus | |
| KCl | slight | Rattus norvegicus | |
| KCN | slight | Rattus norvegicus | |
| LiCl | slight | Rattus norvegicus | |
| Metabisulfite | - |
Rattus norvegicus | |
| MgCl2 | slight | Rattus norvegicus | |
| Na-metaborate | - |
Rattus norvegicus | |
| Na-tetraborate | - |
Rattus norvegicus | |
| Na2SO3 | slight | Rattus norvegicus | |
| Na2SO4 | slight | Rattus norvegicus | |
| NaCl | slight | Rattus norvegicus | |
| NaF | slight | Rattus norvegicus | |
| NaHSO3 | slight | Rattus norvegicus | |
| SDS | - |
Rattus norvegicus | |
| sodium arsenite | slight | Rattus norvegicus | |
| sodium deoxycholate | - |
Rattus norvegicus | |
| sodium taurocholate | - |
Rattus norvegicus | |
| Steroids | overview, e.g. estrone, estradiol, C21 steroids, C19 steroids | Rattus norvegicus | |
| Sulfate esters | overview, e.g. p-nitrophenyl sulfate | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | membrane-bound | Rattus norvegicus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 130000 | - |
gel filtration | Rattus norvegicus |
| 130000 | - |
estrone sulfatase is identical to arylsulfatase C but not to sulfatases which hydrolyse dehydroepiabdrosterone, testosterone, pregnenolone, and cholesterol | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| steroid sulfates + H2O | Rattus norvegicus | also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site | steroid + sulfate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4oC, pH 7.4, imidazol buffer, several months, small loss of activity | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cholesterol 3-sulfate + H2O | - |
Rattus norvegicus | cholesterol + sulfate | - |
? | |
| dehydroandrosterone 3-sulfate + H2O | - |
Rattus norvegicus | dehydroandrosterone + sulfate | - |
? | |
| dehydroepiandrosterone 3-sulfate + H2O | - |
Rattus norvegicus | dehydroepiandrosterone + sulfate | - |
? | |
| estrone sulfate + H2O | - |
Rattus norvegicus | estrone + sulfate | - |
? | |
| additional information | - |
Rattus norvegicus | ? | - |
? | |
| p-nitrophenyl-sulfate + H2O | arylsulfatase activity, EC 3.1.6.1, and various steroid sulfatase activities (EC 3.1.6.1) are functions of the same protein | Rattus norvegicus | p-nitrophenol + sulfate | - |
? | |
| pregnenolone 3-sulfate + H2O | - |
Rattus norvegicus | pregnenolone + sulfate | - |
? | |
| steroid sulfates + H2O | also acts on some related steryl sulfates, phenol sulfates, steroid arylsulfates and steroid alkyl sulfates catalyzed at the same site | Rattus norvegicus | steroid + sulfate | - |
? | |
| testosterone 3-sulfate + H2O | - |
Rattus norvegicus | testosterone + sulfate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | arylsulfatase activity, E.C. 3.1.6.1, and various steroid sulfatase activities, E.C. 3.1.6.1, are functions of the same protein | Rattus norvegicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10 min, 50% loss of activity | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | - |
dehydroepiandrosterone 3-sulfate | Rattus norvegicus |
| 8 | - |
esterone 3-sulfate | Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5: about 25% of activity maximum, pH 8.5: about 15% of activity maximum, dehydroepiandrosterone 3-sulfate | Rattus norvegicus |
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