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Literature summary for 3.1.6.13 extracted from

  • Daniele, A.; Tomanin, R.; Villani, G.R.; Zacchello, F.; Scarpa, M.; Di Natale, P.
    Uptake of recombinant iduronate-2-sulfatase into neuronal and glial cells in vitro (2002), Biochim. Biophys. Acta, 1588, 203-209.
    View publication on PubMed
Search for more literature for 3.1.6.13

Application

Application Comment Organism
medicine the inherited deficiency results in mucopolysaccharidosis type II, the Hunter syndrome, studies of transfer of the recombinant enzyme into brain cells in vitro Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
production of the recombinant enzyme by a transfected human clone: Bosc 23 cells and production of the recombinant enzyme by adenoviral transduction of neuronal cells: SK-N-BE Homo sapiens
production of the recombinant enzyme by adenoviral transduction of glial cells: C6 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000 45000 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is involved in the catabolism of the mucopolysaccharides heparan and dermatan sulfate ?
-
 ?
additional information Rattus norvegicus the enzyme is involved in the catabolism of the mucopolysaccharides heparan and dermatan sulfate ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
glioma cell
-
 Rattus norvegicus
-
kidney
-
 Homo sapiens
-
neuroblastoma cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.006
-
 untreated neuronal cells, comparison to cells after adenoviral infection Homo sapiens
0.00725
-
 untreated control cells, comparison to cells after adenoviral infection Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in the catabolism of the mucopolysaccharides heparan and dermatan sulfate Homo sapiens ?
-
 ?
additional information the enzyme is involved in the catabolism of the mucopolysaccharides heparan and dermatan sulfate Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 42000-45000, SDS-PAGE Homo sapiens