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Literature summary for 3.1.6.13 extracted from

  • Bielicki, J.; Freeman, C.; Clements, P.R.; Hopwood, J.J.
    Human liver iduronate-2-sulphatase. Purification, characterization and catalytic properties (1990), Biochem. J., 271, 75-86.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.6.13

General Stability

General Stability Organism
bovine serum albumin stabilizes Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Homo sapiens
N-actetylglucosamine 6-sulfate-(1-4)-2,5-anhydro-D-mannitol 6-sulfate competitive inhibitor Homo sapiens
NaCl
-
 Homo sapiens
O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol competitive inhibitor Homo sapiens
O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate competitive inhibitor Homo sapiens
O-(alpha-L-idopyranosyluronic acid)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate competitive inhibitor Homo sapiens
phosphate
-
 Homo sapiens
SO42-
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0007 0.0011 O-(alpha-L-iduronic acid 2-sulfate)-(1-3)-2,5-anhydro-D-talitol 4-sulfate pH dependent Homo sapiens
0.0014
-
 O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate pH dependent Homo sapiens
0.0019
-
 O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-O-(beta-D-glucuronic acid or L-iduronic acid)-(1-4)-D-O-(alpha-N-acetylglucosamine)-(1-3)-D-O-gulonic acid pH dependent Homo sapiens
0.0025
-
 O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate pH dependent Homo sapiens
0.0025 0.01 O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate pH dependent Homo sapiens
0.0031
-
 O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha-N-acetylglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate pH dependent Homo sapiens
0.0192
-
 O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates at 2 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
 1 * 42000 + 1 * 18000, SDS-PAGE, reduced Homo sapiens
42000
-
 1 * 42000 + 1 * 18000, SDS-PAGE, reduced Homo sapiens
42000 65000 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dermatan sulfate + H2O Homo sapiens
-
 dermatan + sulfate
-
 ?
heparan sulfate + H2O Homo sapiens
-
 dermatan + sulfate
-
 ?
heparin + H2O Homo sapiens
-
 desulfated heparin + sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Homo sapiens
-
liver
-
 Homo sapiens
-
lung
-
 Homo sapiens
-
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dermatan sulfate + H2O
-
 Homo sapiens dermatan + sulfate
-
 ?
heparan sulfate + H2O
-
 Homo sapiens dermatan + sulfate
-
 ?
heparin + H2O
-
 Homo sapiens desulfated heparin + sulfate
-
 ?
O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol + H2O
-
 Homo sapiens O-(alpha-L-idopyranosyluronic acid)-(1-4)-2,5-anhydro-D-mannitol + sulfate
-
 ?
O-(alpha-L-idopyranosyluronic acid 2-sulfate)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate + H2O
-
 Homo sapiens O-(alpha-L-idopyranosyluronic acid)-(1-4)-2,5-anhydro-D-mannitol 6-sulfate + sulfate
-
 ?
O-(alpha-L-iduronic acid 2-sulfate)-(1-3)-2,5-anhydro-D-talitol 4-sulfate + H2O
-
 Homo sapiens O-(alpha-L-iduronic acid)-(1-3)-2,5-anhydro-D-talitol 4-sulfate + sulfate
-
 ?
O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate + H2O
-
 Homo sapiens O-(alpha-L-iduronic acid)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate + sulfate
-
 ?
O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-O-(beta-D-glucuronic acid or L-iduronic acid)-(1-4)-D-O-(alpha-N-acetylglucosamine)-(1-3)-D-O-gulonic acid + H2O
-
 Homo sapiens O-(alpha-L-iduronic acid)-(1-4)-D-O-(alpha 2-sulfaminoglucosamine 6-sulfate)-(1-4)-O-(beta-D-glucuronic acid or L-iduronic acid)-(1-4)-D-O-(alpha-N-acetylglucosamine)-(1-3)-D-O-gulonic acid + sulfate
-
 ?
O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate + H2O
-
 Homo sapiens O-(alpha-L-iduronic acid)-(1-4)-D-O-(alpha glucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate + sulfate
-
 ?
O-(alpha-L-iduronic acid 2-sulfate)-(1-4)-D-O-(alpha-N-acetylglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid 2-sulfate)-D-O-2,5-anhydro-mannitol 6-sulfate + H2O
-
 Homo sapiens O-(alpha-L-iduronic acid)-(1-4)-D-O (alpha N-acetylglucosamine 6-sulfate)-(1-4)-L-O-(alpha-L-iduronic acid)-D-O-2,5-anhydro-mannitol 6-sulfate + sulfate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 42000 + 1 * 18000, SDS-PAGE, reduced Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9 5.7 depending on substrate Homo sapiens