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Literature summary for 3.1.6.1 extracted from

  • Kowalewski, B.; Luebke, T.; Kollmann, K.; Braulke, T.; Reinheckel, T.; Dierks, T.; Damme, M.
    Molecular Characterization of arylsulfatase G: expression, processing, glycosylation, transport and activity (2014), J. Biol. Chem., 289, 27992-28005.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in HT-1080 cells Mus musculus

Protein Variants

Protein Variants Comment Organism
N215Q residue Asn215 functions as glycosylation site Mus musculus
N356Q residue Asn356 functions as glycosylation site Mus musculus
N497Q mutation of glycosylation site N497 abrogates transport to lysosomes, due to impaired mannose 6-phosphate modification Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome 63-kDa single-chain precursor protein localizes to pre-lysosomal compartments and tightly associates with organelle membranes. Proteolytically processed arylsulfatase G fragments of 34-, 18-, and 10-kDa are found in lysosomal fractions and lost their membrane association. Lysosomal transport of arylsulfatase G in the liver is independent of mannose 6-phosphate, sortilin, and Limp2. Mutation of glycosylation site N497 abrogates transport to lysosomes, due to impaired mannose 6-phosphate modification Mus musculus 5764
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membrane 63-kDa single-chain precursor protein localizes to pre-lysosomal compartments and tightly associates with organelle membranes. Proteolytically processed arylsulfatase G fragments of 34-, 18-, and 10-kDa are found in lysosomal fractions and lost their membrane association Mus musculus 16020
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Organism

Organism UniProt Comment Textmining
Mus musculus Q3TYD4
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
additional information presence of a disulfide bridge between the processed 18-kDa fragment and another smaller polypeptide, probably involving residue Cys195 of the 10-kDa chain Mus musculus
proteolytic modification 63-kDa single-chain precursor protein localizes to pre-lysosomal compartments and tightly associates with organelle membranes. Proteolytically processed arylsulfatase G fragments of 34-, 18-, and 10-kDa are found in lysosomal fractions and lost their membrane association. Proteases participating in the processing are cathepsins B and L. Proteolytic processing is dispensable for hydrolytic sulfatase activity in vitro Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Mus musculus
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heart poor expression Mus musculus
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kidney
-
Mus musculus
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liver
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Mus musculus
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lung poor expression Mus musculus
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spleen
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Mus musculus
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testis
-
Mus musculus
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Synonyms

Synonyms Comment Organism
arylsulfatase G
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Mus musculus