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Literature summary for 3.1.6.1 extracted from
- Arylsulfatase K, a novel lysosomal sulfatase (2013), J. Biol. Chem., 288, 30019-30028.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in HEK-293 cells | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C80A | about 20% of wild-type activity at neutral pH, about 5% of wild-type activity at pH optimum | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Homo sapiens |  |
| sulfate | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.9 | - |
4-nitrocatechol sulfate | pH 4.6, 37°C | Homo sapiens | |
| 20.6 | - |
4-nitrocatechol sulfate | pH 4.6, 37°C | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | protein carries mannose 6-phosphate, indicating lysosomal sorting | Homo sapiens | 5764 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 68000 | - |
x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form | Homo sapiens |
| 70000 | - |
x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q6UWY0 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | when expressed in human cells, isoform ArsK is detected as a 68-kDa glycoprotein carrying at least four N-glycans of both the complex and high-mannose type. Protein carries mannose 6-phosphate, indicating lysosomal sorting | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | mRNA expression is found in all tissues tested | Homo sapiens | - |
| pancreas | high level of expression | Homo sapiens | - |
| placenta | high level of expression | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrocatechol sulfate + H2O | - |
Homo sapiens | 4-nitrocatechol + sulfate | - |
? | |
| 4-nitrophenyl sulfate + H2O | - |
Homo sapiens | 4-nitrophenol + sulfate | - |
? | |
| additional information | activity is dependent on residue cysteine80, which undergoes conversion to formylglycine. No substrate: 4-methylumbelliferyl sulfate | Homo sapiens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 68000, SDS-PAGE of recombinant glycoprotein, cellular form | Homo sapiens |
| ? | x * 70000, SDS-PAGE of recombinant glycoprotein, secreted form | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ARSK | - |
Homo sapiens |
| arylsulfatase K | - |
Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Homo sapiens |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 2.4 | - |
pH 4.6, 37°C | Homo sapiens | phosphate | |
| 2.9 | - |
pH 4.6, 37°C | Homo sapiens | sulfate | |
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Release 2023.1 (January 2023)
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