Cloned (Comment) | Organism |
---|---|
astA gene from Pseudoalteromonas carrageenovora genome is subcloned into pHCEIA vector employing the hyper constitutive expression promoter from the D-amino acid aminotransferase gene of Geobacillus toebii. When the cell is cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reaches 12.8 micromol/min/mL. On MaxyBroth-HD medium with 2% glycerol, the cell shows 2.7fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 micromol/min/mL of arylsulfatase at 20 h, which corresponds to 4fold higher enzyme activity than that of 2% glycerol batch culture | Pseudoalteromonas carrageenovora |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas carrageenovora | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl sulfate + H2O | - |
Pseudoalteromonas carrageenovora | 4-nitrophenol + sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
arylsulfatase gene | - |
Pseudoalteromonas carrageenovora |
AstA | - |
Pseudoalteromonas carrageenovora |