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Literature summary for 3.1.6.1 extracted from
- Purification of a derepressible arylsulfatase from Chlamydomonas reinhardtii. Properties of the enzyme in intact cells and in purified state (1975), Biochim. Biophys. Acta, 384, 168-179.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| imidazole | strongly increases activity | Chlamydomonas reinhardtii |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Borate | - |
Chlamydomonas reinhardtii | |
| CN- | - |
Chlamydomonas reinhardtii | |
| PO43- | no inhibition | Chlamydomonas reinhardtii | |
| SO42- | no inhibition | Chlamydomonas reinhardtii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
4-nitrophenyl sulfate | without imidazole | Chlamydomonas reinhardtii | |
| 0.2 | - |
4-nitrophenyl sulfate | with imidazole | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47000 | - |
1 * 47000, SDS-PAGE | Chlamydomonas reinhardtii |
| 150000 | - |
gel filtration, ultracentrifugation | Chlamydomonas reinhardtii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlamydomonas reinhardtii | - |
type I arylsulfatase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Chlamydomonas reinhardtii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 8 weeks, about 80% loss of activity | Chlamydomonas reinhardtii |
| 3°C, 0.1 M sodium phosphate buffer, 14 days, 25% loss of activity | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl sulfate + H2O | - |
Chlamydomonas reinhardtii | 4-nitrophenol + sulfate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 47000, SDS-PAGE | Chlamydomonas reinhardtii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Chlamydomonas reinhardtii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | 10°C: about 35% of maximal activity, 60°C: about 30% of maximal activity | Chlamydomonas reinhardtii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
rapidly inactivated at alkaline and acid pH, more thermostable at pH 6.5-7.0 | Chlamydomonas reinhardtii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
with imidazole | Chlamydomonas reinhardtii |
| 9.8 | - |
without imidazole | Chlamydomonas reinhardtii |
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